Tristar 2 surface area and porosity analyzer

Benzene-1, 3, 5-tricarboxylic acid was purchased from Aldrich. Copper(II) nitrate hemientahydrate, N,N-dimethylformamide, ethanol and dichloromethane were purchased from Fisher. Bovine serum albumin, casein, trypsin, penicillin, streptomycin and crystal violet were purchased from Sigma. RPMI1640 medium and fetal bovine serum was purchased from Hyclone. All commercial chemicals were used without further purification. Fourier transform infrared measurements were performed on a Nicolet 8700 Instrument. X-ray powder diffraction (XRD) experiments were conducted on a D/max-3B spectrometer with Cu Kα radiation. Scans were made in the 2θ range 3–80° with a scan rate of 10° min⁻¹ (wide angle diffraction). Pore size distributions, BET surface areas and pore volumes were measured by nitrogen adsorption/desorption measurements using a Micromeritics Tristar II Surface area and porosity analyzer. Prior to the analysis, the samples were degassed at 90°C for 1 h. Scanning electron microscopy (SEM) images of the samples were taken on a FEI Quanta 200FEG microscope. Inductively coupled plasma–atomic emission spectrometry (ICP–AES) analysis was used to determine the contents of Cu²⁺ released from Cu-MOF. ICP-AES measurement was carried out with a Shimadzu ICPS-1000IV model. A549 cells visualized using an Olympus IX73 microscope.

Copper dichloride dihydrate (CuCl₂• 2H₂O, 99%), NaOH, polyethylene glycol (PEG, 99%), sodium acetate (NaAc), ferric chloride hexahydrate (FeCl₃•6H₂O, 99%), ethanol, casein, bovine serum albumin (BSA) were acquired from Sigma-Aldrich. Streptomycin and Penicillin were acquired from Sigma Chemical Co. Ltd., (St. Louis, MO, USA). Dimethyl sulfoxide (DMSO) was obtained from Yongda Chemical Reagent Co., Ltd. (Tianjin, China). Fetal bovine serum and DMEM medium were purchased from Hyclone Laboratories (Logan, UT). The powder synchrotron X-ray diffraction patterns were obtained on a Cu Kα radiation in a 2θ range 20–80^° using a D/max-3B spectrometer. Scanning electron microscopy (SEM) images of samples were visualized using a FEI Quanta 200FEG microscope. Transmission electron microscopy (TEM) images of samples were performed on a JEM-2100 microscope. BET surface areas, pore volumes and Pore size distributions were carried out through nitrogen adsorption/desorption measurements using a Micromeritics Tristar II surface area and porosity analyzer. The cells were analyzed using an Olympus IX73 fluorescent microscope (Olympus IX73, Japan) and a FACSCalibur flow cytometer (Becton Dickinson, San Jose, CA.). Images of the western blot were taken with the Champchemi Professional image analysis system (Sagecreation, Beijing, China).