Hek293t

SW620 (#CCL-227), SW480 (#CCL-228), A549 (#CRL-185), PANC1 (#CRL-1469), HeLa(#CCL-2), and RD(#CCL-136) were obtained from the American Type Culture Collection (ATCC). HEK293T (#21573) was obtained from the Korean Cell Line Bank. AMPKγ1 WT or AMPKγ1 KO 293 A cell line was a kind gift of Prof. Hyun Woo Park (Yonsei University, Seoul, Korea). SW620, SW480, and A549 cells were cultured in RPMI-1640 medium (Welgene, #LM011-01) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Welgene, #S001-07) and 1% penicillin-streptomycin (Hyclone, #SV30010) in a 37 °C incubator in a 5% CO₂ atmosphere. HEK293T, PANC1, RD, AMPKγ1 WT 293 A, AMPKγ1 KO 293 A, and HeLa cells were cultured in DMEM medium (Welgene, #LM001-05) supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin in a 37 °C incubator in a 5% CO₂ atmosphere.

MCF10A, ZR75-30, MDA-MB-453, BT20, JIMT-1, MDA-MB-231, and HEK293T cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, T47D, ZR75-1, SKBR3, BT474, HCC-1954, HCC1419, and HS578T cells were purchased from the Korean Cell Line Bank. MCF7, T47D, MDA-MB-231, and HEK293T cells were cultured in DMEM (Welgene, Daegu, Republic of Korea) supplemented with 10% FBS, and SKBR3, BT474, HCC-1954, HCC-1419, JIMT-1, and HS5788T cells were cultured in RPMI (Welgene) supplemented with 10% FBS at 37 °C in a 5% CO2 atmosphere. Trastuzumab was obtained from Roche (Basel, Switzerland). The β-Catenin/TCF inhibitor FH-535 was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Human HNSCC cell lines (UMSCC1, UMSCC4, UMSCC14A, UMSCC38, and OSC19-Luc) were kindly provided by Dr. Jeffrey N. Myers (MD Anderson Cancer Center, Houston, TX, USA). The human NSCLC cell line H226B was kindly provided by Dr. John V. Heymach (MD Anderson Cancer Center). The human HNSCC cell line FADU, human NSCLC cell line H1299, and the human embryonic kidney 293T cell lines (HEK293T) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; for UMSCC1, UMSCC4, UMSCC14A, FADU, OSC19-Luc, and HEK293T cell lines), Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F12; for UMSCC38 cells), or RPMI 1640 medium (for NSCLC cell lines), supplemented with 10% fetal bovine serum (FBS) and antibiotics (all from WelGENE, Kyeongsan-si, Republic of Korea), and maintained at 37 °C with 5% CO₂ in a humidified atmosphere. We used cells that were passaged for fewer than 20 times after resuscitation.