Anti u2af2

Antibodies were purchased from the following companies: anti‐H3K27me1, anti‐H3K27me3, anti‐H3K79me1, anti‐H3K79me2, anti‐histone H3, anti‐hnRNP I, anti‐KDM4A, anti‐KDM5A, anti‐KDM5C, anti‐snRNP70, anti‐tubulin, and anti‐U2AF2 from Abcam (Cambridge, MA, USA); anti‐hnRNP A2/B1 from Acris (San Diego, CA, USA); anti‐SR protein‐specific kinases (SRPK)1 and anti‐SRPK2 from BD Biosciences (San Diego, CA, USA); anti‐AKT, anti‐H3K27me2, anti‐protein phosphatase‐1 (PP1), anti‐phospho‐PP1 at Thr320, anti‐phospho‐AKT at Ser473, and anti‐cleaved‐CASPASE‐3 from Cell Signaling Technology (Billerica, MA, USA); anti‐H3K4me1, anti‐H3K4me2, anti‐H3K4me3, and anti‐H3K36me3 from Millipore; anti‐pro‐CASPASE‐3 and anti‐KDM7A from GeneTex (SanAntonio, TX, USA); anti‐hnRNP C1/C2, anti‐BAX, and anti‐BCL2L1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti‐hnRNP A1 from Sigma; and anti‐SRSF1 and anti‐SRSF3 from Zymed (San Francisco, CA, USA).

Dulbecco’s modified Eagle’s medium (DMEM) containing fetal bovine serum (FBS), penicillin‒streptomycin, and actinomycin D was purchased from Sigma-Aldrich (USA). TRIzol reagent, Lipofectamine 3000, anti-insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) primary antibody, bicinchoninic acid (BCA) kit, electrochemiluminescence (ECL) kit, BODIPY-C11, and SYBR Premix Ex Taq were purchased from Thermo Fisher Scientific (USA). The CCK-8 reagent was purchased from Dojindo Laboratories (Kumamoto, Japan). EdU reagent and RIPA lysis buffer were purchased from Beyotime (Shanghai, China). Anti-solute carrier family 7 member 11 (SLC7A11), anti-m6A, anti-U2AF2, anti-IgG antibodies, and an iron assay kit were purchased from Abcam (Shanghai, China). A fluorescence in situ hybridization (FISH) kit was purchased from RiboBio (Guangzhou, China). The Magna MeRIP m6A kit was purchased from Millipore (USA). The Magnetic RNA Protein Pull-Down Kit was purchased from Pierce (USA).