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Reduced gsh assay kit

Manufactured by Solarbio
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Sourced in China

The Reduced GSH assay kit is a laboratory tool used to quantify the levels of reduced glutathione (GSH) in biological samples. It provides a reliable and accurate method for measuring this important antioxidant molecule.

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Lab products found in correlation

Cat assay kit, by Solarbio (2 mentions) Tg content detection kit, by Solarbio (1 mentions) Sod assay kit, by Solarbio (1 mentions) T aoc assay kit, by Solarbio (1 mentions) Microplate reader, by Tecan (1 mentions)

3 protocols using reduced gsh assay kit

1

Liver Metabolic Profile Analysis

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Approximately 30 mg liver tissue was ground into homogenate to detect triglyceride (TG), free fatty acid (FFA), glutathione (GSH), SOD, and catalase (CAT) levels by TG content detection kit (Cat# BC0625), FFA content detection kit (Cat# BC0595), reduced GSH assay kit (Cat# A006-2-1), SOD assay kit (Cat# A001-3-2), and CAT assay kit (Cat# A007-1-1), which were from Solarbio (Beijing, China) and Nanjing Jiancheng Bioengineering Insititute (Nanjing, China).
Wang M., Zhang F., Zhou J., Gong K., Chen S., Zhu X., Zhang M., Duan Y., Liao C., Han J, & Yin Z. (2023). Glabridin Ameliorates Alcohol-Caused Liver Damage by Reducing Oxidative Stress and Inflammation via p38 MAPK/Nrf2/NF-κB Pathway. Nutrients, 15(9), 2157.
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2

Antioxidant Activity Quantification

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According to the manufacturer’s requirements, 24 h after cell transfection, a T-AOC assay kit (Solarbio), CAT assay kit (Solarbio), and reduced GSH assay kit (Solarbio) were used to detect the levels of T-AOC, CAT, and GSH, respectively. The absorption value was measured with a microplate reader (Tecan, Mannedorf, Switzerland). The T-AOC and activity of GSH and CAT were calculated based on the absorption value and standard curve.
Yu W., Jiang H., Liu F., Li Z., Xu L., Liu C., Lv W., Wang J., Gao Y., Liang S., Kim N.H., Zhang J., Chen C, & Yuan B. (2022). KRAS Affects the Lipid Composition by Regulating Mitochondrial Functions and MAPK Activation in Bovine Mammary Epithelial Cells. Animals : an Open Access Journal from MDPI, 12(22), 3070.
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3

Measuring Colonic Reduced Glutathione

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The levels of reduced GSH in the colon tissues were examined by reduced GSH assay kit (Solarbio, Beijing, China) according to the manufacturer’s instructions. The absorbance value was measured at 412 nm by a microplate reader.
Gao Y., Zhang Z., Du J., Yang X., Wang X., Wen K, & Sun X. (2023). Xue-Jie-San restricts ferroptosis in Crohn’s disease via inhibiting FGL1/NF-κB/STAT3 positive feedback loop. Frontiers in Pharmacology, 14, 1148770.
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