Bouin solution

Manufactured by Merck Group

Sourced in United States, France

Bouin solution is a fixative used in histological and cytological specimen preparation. It is a mixture of picric acid, formaldehyde, and acetic acid. The primary function of Bouin solution is to preserve and harden tissue samples, enabling effective sectioning and staining for microscopic analysis.

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Lab products found in correlation

Masson dye solution set, by Wuhan Servicebio Technology (1 mentions) Microscope dm 2500, by Leica (1 mentions) Weigert s iron haematoxylin, by Merck Group (1 mentions) Infinite m200 pro plate reader, by Tecan (1 mentions) Hematoxylin, by Thermo Fisher Scientific (1 mentions) Anti cd68 antibody ab125212, by Abcam (1 mentions) Goat anti rabbit igg h l alexa fluor 647, by Abcam (1 mentions) Sodium periodate naio4, by Thermo Fisher Scientific (1 mentions) Trichrome stain masson kit, by Merck Group (1 mentions) Infinite m200, by Tecan (1 mentions) Axiocam camera, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Crystal violet, by Tecan (1 mentions) Infinite m200 pro spectrophotometer, by Tecan (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Rabbit anti ucp1, by Abcam (1 mentions) Sigma fast 3 3 diaminobenzidine, by Merck Group (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Hydromount, by National Diagnostics (1 mentions)

Spelling variants of this product

Bouin’s solution (278 citations) Bouin’s fixative solution (16 citations) Preheated Bouin’s solution (2 citations)

22 protocols using bouin solution

Evaluating bHDL and Peptide Therapeutics in Murine Lung Cancer

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BALB/c mice were intravenously injected with 2×10⁴ CT26 cells in 100μL of PBS via tail vein injection and the mice were treated with vehicle (0.15M NaCl and 0.3mM EDTA at pH 7.4) or bHDL (bHDL) at 10mg/kg/day/mouse administered subcutaneously for 3 weeks. After 3 weeks of treatment, the mice were sacrificed; lungs were harvested, weighed, and fixed with Bouin solution (Sigma). Tumor nodules on the lung surface were counted.
For the experiments using peptides, BALB/c mice were intravenously injected with 2×10⁴ CT26 cells in 100μL of PBS via tail vein and the mice were treated starting the same day with the peptide at 100mg/kg/day administered in a chow diet for 3 weeks, or fed with the regular chow diet. After 3 weeks of treatment, the mice were sacrificed; lungs were harvested, weighed, and fixed with Bouin solution (Sigma). Tumor nodules were counted, and lungs were fixed in formalin solution for sectioning. 1cm of jejunum was collected from each mouse to isolate lamina propria and another 1cm of jejunum was collected and fixed in formalin solution for sectioning.

Su F., GM A., Palgunachari M.N., White C.R., Stessman H., Wu Y., Vadgama J., Pietras R., Nguyen D., Reddy S.T, & Farias-Eisner R. (2020). Bovine HDL and Dual Domain HDL-Mimetic Peptides Inhibit Tumor Development in Mice. Journal of cancer research and therapeutic oncology, 8(1), 101.

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Evaluating bHDL and Peptide Therapeutics in Murine Lung Cancer

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Histological Analysis of Heart Tissue

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The heart was excised into segments that were 5 μm long and fixed in paraffin after being fixed in the Bouin solution (4% paraformaldehyde) (Sigma-Merck). After that, the cells were stained following industry protocol using hematoxylin and eosin (SBT10001; Sunteambio Biotechnology, Shanghai, China) and a masson dye solution set (Servicebio).

Liu M., Du X., Chen H., Bai C, & Lan L. (2024). Systemic investigation of di-isobutyl phthalate (DIBP) exposure in the risk of cardiovascular via influencing the gut microbiota arachidonic acid metabolism in obese mice model. Regenerative Therapy, 27, 290-300.

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Histological Analysis of Mouse Tissue

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Mouse tissue was fixed in Bouin solution (Sigma) and stored in 70% ethanol. Tissue staining with hematoxylin and eosin was performed by the University of Virginia Research Histology Core [17 (link)]. Histological scoring was performed by 2 independent blinded scorers as previously described [29 (link)].

Ghosh S., Padalia J., Ngobeni R., Abendroth J., Farr L., Shirley D.A., Edwards T, & Moonah S. (2019). Targeting Parasite-Produced Macrophage Migration Inhibitory Factor as an Antivirulence Strategy With Antibiotic–Antibody Combination to Reduce Tissue Damage. The Journal of Infectious Diseases, 221(7), 1185-1193.

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Quantifying Collagen Content in Heart Tissue

Cited 1 time

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For histochemical analysis of collagen, the Gommori’s trichrome method was used^{41 (link)}. In short, deparaffinated heart tissue sections (n = 10/mouse/time point) were fixated with Bouin’ solution (Sigma-Aldrich, Darmstadt, Germay), followed by Weigert’s iron haematoxylin (Sigma-Aldrich, Darmstadt, Germay) staining and Gomori’s trichrome staining solution. Histological images of 2 sections per mouse and 9 images per section were recorded (40x objective) with Diskus software (Hilgers, Königswinter, Germany) by using Leica Microscope DM 2500 (Leica, Amsterdam, Netherlands) and analyzed by means of commercially available ImageJ software (National Institute of Mental Health, Bethesda, Maryland, USA). Using threshold area fraction determination, the percentage of collagen positive areas was calculated. The amount of collagen was reported as a percentage from the total number of pixels in the optical view as percentage and expressed as mean ± SEM^{40 (link)}. The analysis was blindly performed by two scientists.

Rusu M., Hilse K., Schuh A., Martin L., Slabu I., Stoppe C, & Liehn E.A. (2019). Biomechanical assessment of remote and postinfarction scar remodeling following myocardial infarction. Scientific Reports, 9, 16744.

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Quantifying Biofilm Formation in Bacteria

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The glycerol stocks of the target strains or populations were inoculated in LB medium supplemented with kanamycin (20 µg/mL) for over-day 5 h culture at 37°C, then inoculated in either a polystyrene Greiner 96-well plate or a polyvinyl chloride Corning plate at OD₆₀₀ = 0.01 (5 × 10⁶ cells/mL) in M63B1 minimum medium supplemented with 0.4% glucose and kanamycin. Incubation was done at 37°C for overnight, 16 h. The supernatant was removed from each well and Bouin solution (Sigma) was applied for 15 min to fix the biofilm attached to the well. Next the fixation solution was washed three times with water and biofilm was stained with 1% crystal violet solution (QCA) for 15 min. After removal of the crystal violet solution, biofilms were washed three times with water. For quantification of biofilm formation, dried stained biofilms were resuspended in 30% acetic acid and absorbance was measured at 585 nm using an infinite Tecan infinite M200 PRO plate reader. Biofilm formation in different strains is represented in values normalized to average biofilm formation in the control strains.

Yoshida M., Thiriet-Rupert S., Mayer L., Beloin C, & Ghigo J.M. (2022). Selection for nonspecific adhesion is a driver of FimH evolution increasing Escherichia coli biofilm capacity. microLife, 3, uqac001.

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Histological Quantification of Fibrosis

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Briefly, slides were fixed in Bouin solution (Sigma Aldrich) 15 minutes
at 56 degrees Celsius. After washes in tap water (15 minutes) and deionized
water, slides were stained with Hematoxylin10 minutes (Fisher Scientific). After
5 minutes wash in tap water, slides were dipped in a mix solution of
phosphotungstic acid and phosphomolybdic acid solutions (2.5%) for 5 minutes,
then in Aniline solution 5 minutes and finally in Acetic acid solution (1%) 2
minutes. Slides were washed in deionized water and then dehydrated by Ethanol
95% and 100% bath 2 minutes each. After 2 baths of xylene, slides were mounted
with Cyotseal 60 (Thermo Scientific). 20x magnification pictures were taken
using software Spot v 3.5.7.1 (Diagnostics Instruments, Inc) and fibrosis was
quantified using Image J software (NIH). At least 10 fields per animal were
quantified and at least 4 animals per group.

Bénard L., Oh J.G., Cacheux M., Lee A., Nonnenmacher M., Matasic D.S., Kohlbrenner E., Kho C., Pavoine C., Hajjar R.J, & Hulot J.S. (2016). Cardiac Stim1 Silencing Impairs Adaptive Hypertrophy and Promotes Heart Failure Through Inactivation of mTORC2/Akt Signaling. Circulation, 133(15), 1458-1471.

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Biomaterial Characterization and Evaluation

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Sodium periodate (NaIO₄, >99.8%)
was obtained from Acros Organics (Fair Lawn, NJ). Bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 98% (MTT)
was from Alfa Aesar (Ward Hill, MA). 1X phosphate buffer saline was
from Fisher Scientific Co. (Pittsburgh, PA). Histology mounting medium
polyfreeze, Trichrome Stain (Masson) Kit, bouin solution, and Weiger’s
iron hematoxylin solution were purchased from Sigma-Aldrich (St. Louis,
MO). Anti-S100A4 antibody (ab27957), goat antirabbit IgG H&L (Alexa
Fluor 488) (ab150077), anti-CD68 antibody (ab125212), and goat antirabbit
IgG H&L (Alexa Fluor 647) were purchased from Abcam (Cambridge,
MA). 4′,6-Diamidino-2-phenylindole (DAPI) was obtained from
Invitrogen (Grand Island, NY). Laponite XLG (Laponite) was a gift
from Southern Clay Products, Inc. (Austin, TX). PEG-D4 was prepared
as previously described.³⁶

Liu Y., Meng H., Konst S., Sarmiento R., Rajachar R, & Lee B.P. (2014). Injectable Dopamine-Modified Poly(ethylene glycol) Nanocomposite Hydrogel with Enhanced Adhesive Property and Bioactivity. ACS Applied Materials & Interfaces, 6(19), 16982-16992.

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Embryo Extraction and Preservation

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Pregnant female mice were euthanized by means of cervical dislocation at day 8.5–12.5 post coitus (dpc). The uteruses of the mice were dissected and embryos were collected. Embryos were either snap-frozen in liquid nitrogen for RNA isolation or fixated in Bouin solution (Sigma-Aldrich) for 2 hours and then incubated in 70% ethanol and embedded in paraffin for histological examination.

Renard M., Trachet B., Casteleyn C., Campens L., Cornillie P., Callewaert B., Deleye S., Vandeghinste B., van Heijningen P.M., Dietz H., De Vos F., Essers J., Staelens S., Segers P., Loeys B., Coucke P., De Paepe A, & De Backer J. (2014). Absence of Cardiovascular Manifestations in a Haploinsufficient Tgfbr1 Mouse Model. PLoS ONE, 9(2), e89749.

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Quantification of Collagen in NHDF Cells

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After infection, the NHDF cells
were washed 3 × with PBS and then incubated with Bouin solution
(Sigma-Aldrich) at RT for 20 min. The cells were incubated with 0.1%
Picrosirius red dye (ab150681) at RT for 2 h. Then, they were washed
1 × with 0.01 N HCl and the matrix was dissolved in 0.01 N NaOH.
The absorption was measured at 570 nm using a Tecan Infinite M200
plate reader (Tecan, Grödig, Austria). By dividing the absorbance
of each sample by the absorbance of the healthy sample, the relative
collagen quantity was determined. For each condition, the experiment
was performed three times.

Alhayek A., Abdelsamie A.S., Schönauer E., Camberlein V., Hutterer E., Posselt G., Serwanja J., Blöchl C., Huber C.G., Haupenthal J., Brandstetter H., Wessler S, & Hirsch A.K. (2022). Discovery and Characterization of Synthesized and FDA-Approved Inhibitors of Clostridial and Bacillary Collagenases. Journal of Medicinal Chemistry, 65(19), 12933-12955.

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