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Ghost violet 450 viability dye

Manufactured by Cytek Biosciences
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The Ghost™ Violet 450 viability dye is a fluorescent reagent used to identify and exclude non-viable cells from flow cytometry analyses. It functions by staining dead or membrane-compromised cells, allowing for their discrimination from live, viable cells.

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Lab products found in correlation

Dynabeads sheep anti rat igg, by Thermo Fisher Scientific (1 mentions) Anti cd31, by BioLegend (1 mentions) Anti ter119, by BioLegend (1 mentions) Cd16 32 antibody, by Thermo Fisher Scientific (1 mentions) Anti cd45, by BioLegend (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions)

3 protocols using ghost violet 450 viability dye

1

Isolation and Immunophenotyping of Mammary Epithelial Cells

Cited 2 times
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MECs were lineage-depleted utilizing anti-CD31 (0.5mg/mL,102401; RRID:AB_312896), anti-TER-119 (0.5mg/mL,116201; RRID:AB_313702), and anti-CD45 (0.5mg/mL, 103102; RRID:AB_312967) antibodies (Biolegend) conjugated to sheep anti-rat IgG Dynabeads (Invitrogen, 11035). Lineage-depleted cells were stained with Ghost Violet 450 viability dye (TONBO Biosciences, 13–0863-T100). For immunotyping, MECs were incubated with CD16/32 antibodies (ThermoFisher; 14–0161-82; RRID:AB_467133), followed by fixable viability dye eFluor 780 (eBiosciences; 65–0865). Antibodies are listed in Table S1. Cells were analyzed using BD LSR Fortessa (BD Biosciences). Immune cells were identified as described (21 (link)). Gates were set on fluorescence minus one controls, and data were analyzed using FlowJo software (TreeStar V10; RRID:SCR_008520). Progenitor assays were conducted as described (5 (link)).
Chamberlin T., Clack M., Silvers C., Kuziel G., Thompson V., Johnson H, & Arendt L.M. (2020). Targeting obesity-induced macrophages during preneoplastic growth promotes mammary epithelial stem/progenitor activity, DNA damage and tumor formation. Cancer research, 80(20), 4465-4475.
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2

Th17 Cell Differentiation Assay

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Naïve T helper cells were cultured at 1 × 106/ml in a Th17-polarizating condition for 48 h. Cells were stained for a viability test with Ghost™ Violet 450 viability dye (Tonbo Biosciences; San Diego, CA) and anti-CD4 followed by fixation in 70% ethanol, treatment with ribonuclease, propidium iodide (PI) staining, and analysis by flow cytometry.
Friesen L.R., Gu B, & Kim C.H. (2020). A ligand-independent fast function of RARα promotes exit from metabolic quiescence upon T cell activation and controls T cell differentiation. Mucosal immunology, 14(1), 100-112.
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3

Th17 Cell Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve T helper cells were cultured at 1 × 106/ml in a Th17-polarizating condition for 48 h. Cells were stained for a viability test with Ghost™ Violet 450 viability dye (Tonbo Biosciences; San Diego, CA) and anti-CD4 followed by fixation in 70% ethanol, treatment with ribonuclease, propidium iodide (PI) staining, and analysis by flow cytometry.
Friesen L.R., Gu B, & Kim C.H. (2020). A ligand-independent fast function of RARα promotes exit from metabolic quiescence upon T cell activation and controls T cell differentiation. Mucosal immunology, 14(1), 100-112.
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