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Quantikine mouse leptin immunoassay kit

Manufactured by R&D Systems
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Sourced in Israel

The Quantikine® Mouse Leptin Immunoassay kit is a solid-phase ELISA designed to measure mouse leptin levels in cell culture supernates, serum, and plasma. It is a quantitative assay with a dynamic range of 78.1-5,000 pg/mL.

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Lab products found in correlation

Mouse insulin elisa kit, by Crystal Chem (3 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (1 mentions)

Close spelling variants of this product

Quantikine ELISA mouse Leptin Immunoassay kit Mouse leptin immunoassay kit Leptin immunoassay kit Quantikine Immunoassay kits Quantikine Immunoassays Quantikine kits Leptin Quantikines

4 protocols using quantikine mouse leptin immunoassay kit

1

Plasma Metabolite Quantification Protocol

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After overnight fasting, plasma was prepared from anticoagulated (K2EDTA) whole blood by centrifugation at 2000 g for 15 min. Plasma insulin, adiponectin, and leptin levels were measured using a Mouse Insulin ELISA kit (Crystal Chem Inc., Downers Grove, IL), Quantikine® Mouse Leptin Immunoassay kit, and Quantikine® Mouse Leptin Immunoassay kit (R&D Systems, Minneapolis, MN) as instructed by the manufacturer.
Zhong J., Allen K., Rao X., Ying Z., Braunstein Z., Kankanala S.R., Xia C., Wang X., Bramble L.A., Wagner J.G., Lewandowski R., Sun Q., Harkema J.R, & Rajagopalan S. (2016). Repeated Ozone Exposure Exacerbates Insulin Resistance And Activates Innate Immune Response In Genetically Susceptible Mice. Inhalation toxicology, 28(9), 383-392.
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2

Plasma Biomarker Quantification

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After 6-hour fasting, plasma was prepared from anti-coagulated whole blood by centrifugation at 2000 g for 15 min. Plasma insulin, adiponectin, and leptin levels were measured using a Mouse Insulin ELISA kit (Crystal Chem Inc., Downers Grove, IL), Quantikine® Mouse Leptin Immunoassay kit, and Quantikine® Mouse Leptin Immunoassay kit (R&D Systems, Minneapolis, MN) as instructed by the manufacturer.
Ying Z., Allen K., Zhong J., Chen M., Williams K.M., Wagner J.G., Lewandowski R., Sun Q., Rajagopalan S, & Harkema J.R. (2016). Subacute Inhalation Exposure to Ozone Induces Systemic Inflammation but not Insulin Resistance in a Diabetic Mouse Model. Inhalation toxicology, 28(4), 155-163.
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3

Insulin Resistance Measurement Using HOMA-IR

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Plasma concentrations of insulin, leptin, and adiponectin were determined using a Mouse Insulin ELISA kit (Crystal Chem Inc.), the Quantikine Mouse Leptin Immunoassay kit, and Quantikine Mouse Adiponectin kit (R&D Systems), per the manufacturers’ instructions. Insulin resistance was determined using the homeostatic model assessment of insulin resistance (HOMA-IR), which assesses the ratio of fasting blood glucose and insulin and is routinely used for human clinic assessment (Brook et al. 2013 (link)). HOMA-IR was calculated as follows:
[glucose (mMol/L)×insulin (μU/mL)]/22.5.
Wagner J.G., Barkauskas C.E., Vose A., Lewandowski R.P., Harkema J.R, & Tighe R.M. (2020). Repetitive Ozone Exposures and Evaluation of Pulmonary Inflammation and Remodeling in Diabetic Mouse Strains. Environmental Health Perspectives, 128(11), 117009.
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4

Insulin and Leptin Quantification

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Serum insulin level and leptin level were measured using an ultra-sensitive mouse insulin ELISA kit (Crystal Chem, Inc.) and a quantikine mouse leptin immunoassay kit (R&D Systems) as instructed by the manufacturer.
Zhong J., Rao X., Braunstein Z., Taylor A., Narula V., Hazey J., Mikami D., Needleman B., Rutsky J., Sun Q., Deiuliis J.A., Satoskar A.R, & Rajagopalan S. (2014). T-Cell Costimulation Protects Obesity-Induced Adipose Inflammation and Insulin Resistance. Diabetes, 63(4), 1289-1302.
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