Il 10 apc and il 4 percp cy5

Manufactured by BioLegend

IL-10-APC and IL-4-PerCP-Cy5.5 are fluorochrome-conjugated antibodies used for the detection and analysis of interleukin-10 (IL-10) and interleukin-4 (IL-4) proteins, respectively, in flow cytometry experiments. IL-10 is an anti-inflammatory cytokine, while IL-4 is a Th2 cytokine involved in the regulation of immune responses. These antibodies allow for the specific identification and quantification of cells expressing these cytokines.

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Lab products found in correlation

Permeabilization wash buffer, by BD (2 mentions) Il 1β pe, by Thermo Fisher Scientific (2 mentions) Tnf α pe cy7, by Thermo Fisher Scientific (2 mentions) Fixation permeabilization solution, by BD (2 mentions) Fc block, by BD (2 mentions) Cytoflex, by Beckman Coulter (1 mentions)

Close spelling variants of this product

IL-10 (167 citations) PerCP-Cy5.5 (133 citations) IL-4 APC (9 citations) IL-10-APC (7 citations) IL-10-PerCP-Cy5.5 (3 citations)

2 protocols using il 10 apc and il 4 percp cy5

Ex vivo intracellular cytokine staining

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For intracellular cytokine staining, an ex vivo brefeldin A protocol was followed. Prior to staining, brain leukocytes were incubated with BFA (10 μg/mL, Sigma) in 1 mL complete RPMI for 2 h at 37 °C (5% CO₂). Afterward, cells were resuspended in Fc Block, stained for surface antigens and washed in 100 μL of fixation/permeabilization solution (BD Biosciences) for 20 min. Cells were then washed twice in 300 μL permeabilization/wash buffer (BD Biosciences), resuspended in an intracellular antibody cocktail (0.25 μg for each antibody, 1:100 dilution) containing TNFα‐PE‐Cy7 (eBioscience) and IL‐1β‐PE (eBioscience), IL‐10‐APC and IL‐4‐PerCP‐Cy5.5 (BioLegend) and subsequently fixed.

Al Mamun A., Chauhan A., Yu H., Xu Y., Sharmeen R, & Liu F. (2018). Interferon regulatory factor 4/5 signaling impacts on microglial activation after ischemic stroke in mice. The European Journal of Neuroscience, 47(2), 140-149.

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Ex Vivo Intracellular Cytokine Staining

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For intracellular cytokine staining, an ex vivo brefeldin A protocol was followed. Prior to staining, brain leukocytes (1×10⁶ cells) were incubated with BFA (10 μg/mL, Sigma) in 1mL complete RPMI for 2h at 37 °C (5 % CO2). Afterward, cells were re-suspended in Fc Block, stained for surface antigens and washed in 100μL of fixation/permeabilization solution (BD Biosciences) for 20 minutes. Cells were then washed twice in 300μL permeabilization/wash buffer (BD Biosciences), re-suspended in an intracellular antibody cocktail (0.25μg for each antibody, 1:100 dilution) containing TNFα-PE-Cy7 (eBioscience) and IL-1β-PE (eBioscience), IL-10-APC and IL-4-PerCP-Cy5.5 (BioLegend), and subsequently fixed. Data were acquired on a CytoFLEX (Beckman Coulter) and analyzed with FlowJo (Treestar Inc.).

Mamun A.A., Yu H., Mirza M.A., Romana S., McCullough L.D, & Liu F. (2018). Myeloid Cell IRF4 signaling protects neonatal brains from hypoxic ischemic encephalopathy. Neurochemistry international, 127, 148-157.

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