Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
Gata3
Manufactured by Bio-Rad
GATA3 is a DNA-binding protein that functions as a transcription factor. It plays a crucial role in the development and differentiation of various cell types, particularly in the immune system and certain epithelial tissues. GATA3 regulates the expression of genes involved in these processes.
Automatically generated - may contain errors
Lab products found in correlation
F4 80, by Bio-Rad (2 mentions)
Mouse anti mrc 1, by Abcam (2 mentions)
Phospho stat1 tyr 701 alexa fluor 488 conjugated rabbit antibody, by Cell Signaling Technology (2 mentions)
Gallios flow cytometer, by Cell Signaling Technology (2 mentions)
Alexa fluor 488 goat anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions)
Cd11b, by Bio-Rad (2 mentions)
2 protocols using gata3
1
Multiparametric Immune Profiling by Flow Cytometry
2
Multiparametric Immune Profiling by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!