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Human cxcl10 ip 10 duoset elisa kit

Manufactured by R&D Systems

The Human CXCL10/IP-10 DuoSet ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of human CXCL10/IP-10 concentrations in cell culture supernates, serum, and plasma.

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3 protocols using human cxcl10 ip 10 duoset elisa kit

1

Quantifying CXCL10 and HIV-1 Gag

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CXCL10 was measured in cell-free supernatants by Human CXCL10/IP-10 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). HIV-1 Gagp24 was measured in cell-free supernatants lysed with 0.5% Triton X-100 in PBS by HIV-1 Gagp24 ELISA kit according to the manufacturer’s instructions (Sino Biological, Beijing, China).
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2

Quantification of CXCL10 and HIV-1 Gag

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CXCL10 was measured in cell‐free supernatants by Human CXCL10/IP‐10 DuoSet ELISA kit according to the manufacturer's instructions (R&D Systems, Minneapolis, MN). HIV‐1 Gagp24 was measured in cell‐free supernatants lysed with 0.5% Triton X‐100 in PBS by HIV‐1 Gagp24 ELISA kit according to the manufacturer's instructions (Sino Biological, Beijing, China).
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3

Cytokine Quantification in Frozen Supernatant

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The cell culture supernatant was frozen at −80 °C until use for the analysis.
The levels of VEGF-A and CXCL-10 were measured using a Human VEGFA DuoSet ELISA kit (R&D Systems Inc., DY293B) and Human CXCL10/IP-10 DuoSet ELISA kit (R&D Systems Inc., DY266), respectively.
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