Human THP-1 cells were obtained from American Type Culture Collection (ATCC) and maintained in Roswell Park Memorial Institute (RPMI)-1640 medium (Corning) supplemented with 10% (v/v) of super calf serum (GEMcell) and 2 mM of L-glutamine (Corning) at 37°C and 5% CO2. Human HeLa cells were obtained from ATCC and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% super calf serum (GEMcell) and 2 mM L-glutamine (Corning) at 37°C and 5% CO2. Male SV20 induced pluripotent stem cells were obtained from the University of Pennsylvania Human Pluripotent Stem Cell Core and differentiated over 7 days in PSC Neural Induction Medium (GIBCO) and expanded for 6 days in Neural Expansion Medium (GIBCO) at 37°C and 5% CO2. THP1 cells were treated with 2 μM flavopiridol (Sigma) for 2 h or 5 μg/ml of LPS (Invitrogen) for 5, 15, and 30 min or 4 h in growing medium. HeLa were treated with 100 ng/ml of rEGF (Invitrogen) for 15 min in growing medium.
Super calf serum
Manufactured by Corning
Super calf serum is a cell culture medium supplement derived from the blood of calves. It provides essential nutrients, growth factors, and other components to support the growth and proliferation of a variety of cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Flavopiridol, by Merck Group (2 mentions)
Psc neural induction medium, by Thermo Fisher Scientific (2 mentions)
Neural expansion medium, by Thermo Fisher Scientific (2 mentions)
Lipopolysaccharides (lps), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Corning (2 mentions)
Rpmi 1640 medium, by Corning (2 mentions)
2 protocols using super calf serum
1
Culturing and Treating Cell Lines
2
Culturing and Treating Cell Lines
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Human THP-1 cells were obtained from American Type Culture Collection (ATCC) and maintained in Roswell Park Memorial Institute (RPMI)-1640 medium (Corning) supplemented with 10% (v/v) of super calf serum (GEMcell) and 2 mM of L-glutamine (Corning) at 37°C and 5% CO2. Human HeLa cells were obtained from ATCC and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% super calf serum (GEMcell) and 2 mM L-glutamine (Corning) at 37°C and 5% CO2. Male SV20 induced pluripotent stem cells were obtained from the University of Pennsylvania Human Pluripotent Stem Cell Core and differentiated over 7 days in PSC Neural Induction Medium (GIBCO) and expanded for 6 days in Neural Expansion Medium (GIBCO) at 37°C and 5% CO2. THP1 cells were treated with 2 μM flavopiridol (Sigma) for 2 h or 5 μg/ml of LPS (Invitrogen) for 5, 15, and 30 min or 4 h in growing medium. HeLa were treated with 100 ng/ml of rEGF (Invitrogen) for 15 min in growing medium.
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