Dneasy rneasy isolation kits

DNA and RNA were extracted from a portion of pulverized frozen quadriceps muscle homogenate using DNeasy/RNeasy Isolation Kits (Qiagen, Germantown, MD, USA) as described by the manufacturer. Isolated DNA and RNA were tested for concentration and purity using a NanoDrop Spectrophotometer (Thermo Scientific, Waltham, MA, USA). Isolated RNA was converted into cDNA, assessed for purity, and qPCR of the resulting cDNA levels was performed as previously described (Drew et al., 2014). All genes were normalized to the housekeeping gene Ppia or 18S. Mitochondrial DNA content was assessed as a ratio of mitochondrial DNA (mtCO2) to nuclear DNA (18S). Primers used for qPCR can be found in Table S2.

DNA and RNA were extracted from a portion of pulverized frozen muscle using DNeasy/RNeasy Isolation kits (Qiagen, Germantown, MD, United States) as described by the manufacturer. Isolated DNA and RNA were tested for concentration and purity using a NanoDrop Spectrophotometer (Thermo Scientific, Waltham, MA, United States). Isolated RNA was converted into cDNA, assessed for purity, and qPCR of the resulting cDNA levels was performed as previously described (Drew et al., 2014 (link)). All genes were normalized to the housekeeping genes Ppia or 18S. Mitochondrial DNA content was assessed as a ratio of mitochondrial DNA (mtCO2) to nuclear DNA (18S). Primers used for qPCR can be found in Supplementary Table S1.