Two flow cytometry tubes containing single-cell suspensions (from bone marrow cells, tumor or spleen) in cell staining buffer (CSB; cat. no. 00-4222-57; Invitrogen; Thermo Fisher Scientific, Inc.) were prepared per sample, one serving as the negative control. Each tube contained ~1×106 cells, and 400 µl CSB solution was added to the tube 1 that was stored on ice. Mouse Fc Block (2 µl; BD Biosciences) was added to the tube 2 of each sample. Then, APC rat anti-mouse CD11b (1 µl; BD Pharmingen; BD Biosciences; cat. no. 557657) and PE rat anti-mouse Ly-6G and Ly-6C (1 µl; BD Pharmingen; BD Biosciences; cat. no. 561084) were added to the tube 2 of each sample. After incubation on ice for 20 min in the dark, cells were washed twice with CSB solution, diluted in 500 µl CSB and analyzed by flow cytometry (BD FACSAria III; BD Biosciences) and data were analyzed by Flowjo (v10.0.7; BD Biosciences).
Pe rat anti mouse ly 6g and ly 6c
Manufactured by BD
Sourced in United States
The PE rat anti-mouse Ly-6G and Ly-6C is a flow cytometry reagent designed to detect the Ly-6G and Ly-6C markers on mouse cells. It is conjugated with the fluorescent dye, phycoerythrin (PE).
Automatically generated - may contain errors
2 protocols using pe rat anti mouse ly 6g and ly 6c
1
Immunophenotyping of Immune Cells by Flow Cytometry
2
MSU-Induced Peritonitis for NLRP3 Inflammasome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze NLRP3-induced inflammasome in vivo, MSUinduced peritonitis mouse model was performed. C57BL/6 mice were injected ip with 1 mg MSU in 200 μL of PBS and the control mice received PBS. The peritoneal lavage samples were obtained 5 hours after MSU injection by washing the peritoneal cavity with 2 mL of cold PBS with 1% of FBS. Peritoneal samples were centrifuged and total cells were counted. Subsequently, cells were stained with CD11b (Percp-cy5.5 rat anti-mouse CD11b, BD Pharmingen, 550993), F4/80 (Alexa Fluor 647 rat anti-mouse F4/80, BD Pharmingen, 565853) and Gr-1 (PE rat anti-mouse LY-6G and LY-6C, BD Pharmingen, 553128) antibodies and analyzed using flow cytometry (FACS Calibur (Becton Dickinson, San Diego, CA, USA). The number of neutrophils was calculated as total cells multiplied by CD11b+/Gr-1+/ F4/80-cell ratio. The supernatant of peritoneal lavage fluid
was used to evaluate IL-1β production using Mouse IL-1β ELISA Kit (Abclonal, Wuhan, China).
was used to evaluate IL-1β production using Mouse IL-1β ELISA Kit (Abclonal, Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!