Mip 1α

Manufactured by Eve Technologies

Sourced in Canada

MIP-1α is a chemokine protein that plays a role in the immune system. It functions as a chemoattractant, recruiting and activating specific types of white blood cells to sites of inflammation or infection.

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Lab products found in correlation

5 protocols using mip 1α

Synovial Fluid Cytokine and Adipokine Profiling

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The right knee joints were opened to collect synovial fluid shortly after sacrifice using the Whatman chromatography paper method^{52 (link)}. Samples were weighed, diluted and centrifuged^{40 (link)}. Serum and synovial fluid cytokines and adipokines were quantified using a Rat 27 Multiplex Discovery Assay with Luminex®xMAP technology (Eotaxin, EGF, Fractalkine, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, IP-10/CXCL10, GRO/KC, IFN-γ, TNF-α, G-CSF, GM-CSF, MCP-1, leptin, LIX, MIP-1α, MIP-2, RANTES, VEGF; Eve Technologies, AB, Canada).

Rios J.L., Bomhof M.R., Reimer R.A., Hart D.A., Collins K.H, & Herzog W. (2019). Protective effect of prebiotic and exercise intervention on knee health in a rat model of diet-induced obesity. Scientific Reports, 9, 3893.

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Multiplex Serum Cytokine Analysis in Fasted Rats

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Following 12 h of fasting, rats were anesthetized with isoflurane and a blood sample was collected by cardiac puncture. Blood was centrifuged at 3000 rpm for 15 min at 4 °C and serum stored in aliquots at − 80 °C until analyzed. Rats were sacrificed by heart excision. Serum cytokines and adipokines were quantified using a Rat 27 Multiplex Discovery Assay with Luminex® xMAP technology (eotaxin, EGF, fractalkine, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, IP-10/CXCL10, GRO/KC, IFN-γ, TNF-α, G-CSF, GM-CSF, MCP-1, leptin, LIX, MIP-1α, MIP-2, RANTES, VEGF; Eve Technologies, Calgary, AB, Canada).

Rios J.L., Boldt K.R., Mather J.W., Seerattan R.A., Hart D.A, & Herzog W. (2018). Quantifying the Effects of Different Treadmill Training Speeds and Durations on the Health of Rat Knee Joints. Sports Medicine - Open, 4, 15.

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Multiplex Cytokine Profiling of Cell Supernatants

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Cell supernatants were collected and frozen down at -80 °C until assayed using a Human 42 Multiplex Discovery Assay with Luminex xMAP technology (EGF, Eotaxin-1, FGF-2, Flt-3L, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-18, IP-10, MCP-1, MCP-3, MDC, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, sCD40L, TGFα, TNFα, TNFβ, VEGF-A; Eve Technologies, AB, Canada). The level of VEGF, IL-6, and PGE2 in cell culture supernatants were also evaluated using ELISA (Human DuoSet ELISA Development Kits (VEGF, IL-6, PGE2), R&D Systems) with absorbance measured at 450 nm/570 nm.

Allen A., Vaninov N., Li M., Nguyen S., Igo P., Tilles A.W., O’Rourke B., Miller B.L., Parekkadan B, & Barcia R.N. (2020). Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells. Scientific Reports, 10, 10142.

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Multiplex Cytokine Analysis of Ascites and Plasma

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Ascites fluid and plasma samples collected 24 h following initial STING agonist dose or post carboplatin chemotherapy alone from each treatment group were subjected to multiplex cytokine analysis using the mouse Cytokine Array/Chemokine Array 31-Plex Discovery Assay (includes eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor (GM-CSF), IFN-γ, interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10 (CXCL10), CXCL1, LIF, LIX, MCP-1 (CCL2), macrophage colony-stimulating factor (M-CSF), MIG (CXCL9), MIP-1α, MIP-1β, MIP-2, RANTES (CCL5), tumor necrosis factor-α, and VEGF) at Eve Technologies (Alberta, Canada). All samples were analyzed in biological triplicates. The standard curve regression was used to calculate the concentration of each target cytokine.

Shakfa N., Li D., Conseil G., Lightbody E.D., Wilson-Sanchez J., Hamade A., Chenard S., Jawa N.A., Laight B.J., Afriyie-Asante A., Tyryshkin K., Koebel M, & Koti M. (2023). Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer. Journal for Immunotherapy of Cancer, 11(4), e006170.

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Gastric Cytokine Profiling in Mice

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Gastric tissue from male mice was homogenized in liquid nitrogen with a disposable pestle (Sigma-Aldrich, St. Louis, MO). One hundred fifty microliters of lysis buffer, 500 µL of RIPA buffer with protease inhibitor (Thermo Fisher Scientific, Waltham, MA), 5-µL protease inhibitor, and 5-µL 0.5 M EDTA were added. Samples were placed in a rotating mixer at 4°C for 1 hour. Supernatant was collected following centrifugation at 10,000 × g for 10 minutes at 4°C. Protein concentration was measured using a BCA kit (Thermo Fisher Scientific, Waltham, MA) and adjusted to 1 mg/mL. Thirty-two-plex gastric tissue cytokine array was performed to quantify eotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, and VEGF-A (Eve Technologies, Calgary, Alberta, Canada).

Lunger C., Shen Z., Holcombe H., Mannion A.J., Dzink-Fox J., Kurnick S., Feng Y., Muthupalani S., Carrasco S.E., Wilson K.T., Peek R.M., Piazuelo M.B., Morgan D.R., Armijo A.L., Mammoliti M., Wang T.C, & Fox J.G. (2023). Gastric coinfection with thiopeptide-positive Cutibacterium acnes decreases FOXM1 and pro-inflammatory biomarker expression in a murine model of Helicobacter pylori-induced gastric cancer. Microbiology Spectrum, 12(1), e03450-23.

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