Srebp1 antibody

Manufactured by BD

Sourced in United States

The SREBP1 antibody is a laboratory reagent used to detect and study the SREBP1 protein, a transcription factor involved in the regulation of lipid and cholesterol metabolism. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to analyze the expression and localization of SREBP1 in biological samples.

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Lab products found in correlation

T0901317, by Merck Group (1 mentions) Goat anti mouse secondary antibody, by Santa Cruz Biotechnology (1 mentions) β actin antibody, by Merck Group (1 mentions) Insulin, by Novo Nordisk (1 mentions) Betulin, by Merck Group (1 mentions) Fasn antibody, by Santa Cruz Biotechnology (1 mentions) Acc antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 donkey anti mouse a32766, by Thermo Fisher Scientific (1 mentions) Pf 429242, by Merck Group (1 mentions) γ tubulin antibody, by Merck Group (1 mentions) Srebp2 antibody, by R&D Systems (1 mentions)

3 protocols using srebp1 antibody

Molecular Mechanisms of Cholesterol Regulation

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Cilostazol was donated by the Otsuka Pharmaceutical Co. (Otsuka, Japan). Insulin was provided by Novo Nordisk (Bagsvaerd, Denmark). Radiochemicals ([γ-³²P]ATP) were purchased from Perkin Elmer (Boston, MA, USA). T0901317 was from Sigma (St Louis, MO, USA). The SREBP-1 antibody was purchased from BD Biosciences (San Jose, CA, USA) and the β-actin antibody was purchased from Sigma. The goat anti-mouse secondary antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Jung Y.A., Kim H.K., Bae K.H., Seo H.Y., Kim H.S., Jang B.K., Jung G.S., Lee I.K., Kim M.K, & Park K.G. (2014). Cilostazol inhibits insulin-stimulated expression of sterol regulatory binding protein-1c via inhibition of LXR and Sp1. Experimental & Molecular Medicine, 46(1), e73-.

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Lipogenesis Inhibitors SREBP1, FASN, and ACC

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The SREBP1 inhibitors, PF429242 and betulin, were purchased from Sigma Aldrich (St. Louis, MO) and Merck (Kenilworth, NJ), respectively. SREBP1 antibody was purchased from BD Biosciences (#557036; San Jose, CA). FASN antibody was purchased from Santa Cruz Biotechnology (SC-55580; Santa Cruz, CA). ACC antibody was purchased from Cell Signaling Technology, Inc (#3662; Beverly, MA). Nile Red (N1142), DAPI (62248) and the secondary antibodies, Alexa Fluor 488-Donkey anti-mouse (A32766) and Alexa Fluor 568-Donkey anti-rabbit (A10042) were purchased from Thermo Fisher Scientific (Waltham, MA). Other reagents and antibodies were the same as described previously^{13 (link), 23 (link)}.

Chen Z., Yu D., Owonikoko T.K., Ramalingam S.S, & Sun S.Y. (2021). Induction of SREBP1 degradation coupled with suppression of SREBP1-mediated lipogenesis impacts the response of EGFR mutant NSCLC cells to osimertinib. Oncogene, 40(49), 6653-6665.

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Western Blot Analysis of SREBP

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Colon cancer cells were harvested and detergent-solubilized cell lysates were obtained as described previously^{41 (link)–44 (link)}. Equal amounts of cell lysates were resolved by SDS-PAGE and subjected to western blot analysis. The SREBP1 antibody was from BD Biosciences (CA, USA), whereas the SREBP2 antibody was from R&D Systems (MN, USA). Note that the SREBP antibodies recognize both the precursor and mature active forms of the respective proteins. The γ-tubulin antibody was from Sigma-Aldrich.

Wen Y.A., Xiong X., Zaytseva Y.Y., Napier D.L., Vallee E., Li A.T., Wang C., Weiss H.L., Evers B.M, & Gao T. (2018). Downregulation of SREBP inhibits tumor growth and initiation by altering cellular metabolism in colon cancer. Cell Death & Disease, 9(3), 265.

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