Pcdna3.1 control

Manufactured by GenePharma

Sourced in China

PcDNA3.1-control is a plasmid vector commonly used in molecular biology research. It serves as a control for gene expression studies, providing a baseline for comparison against experimental samples.

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Lab products found in correlation

Pcdna3.1 pten, by GenePharma (1 mentions) Lipofectaminetm 3000 reagent, by Thermo Fisher Scientific (1 mentions) Mirna mimics control mimics nc, by GenePharma (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Effectene transfection reagent, by Qiagen (1 mentions) Pcdna3.1 runx2, by GenePharma (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

4 protocols using pcdna3.1 control

Suppressing SOX2-OT Expression in BEAS-2B Cells

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To suppress SOX2-OT expression, antisense oligonucleotides, ASOs (small single-stranded nucleic acids that bind to SOX2-OT inside the cells), targeting SOX2-OT were designed and synthesized by RiboBio company (Guangzhou, China).
When BEAS-2B cells plated into 6-well plates or 24-well plates grew to 80% confluence, they were transfected with SOX2-OT-targeting ASO (ASO-SOX2OT), negative control ASO (ASO-NC), negative control miR (miR-NC), miR-455-3p mimic (miR-mimic), miR-455-3p inhibitor (miR-inhibitor), pcDNA3.1-control (pcDNA-Con) or pcDNA3.1-PTEN (Genepharma Co.Ltd., Shanghai, China) by Lipofectamine^TM 3000 reagent (Invitrogen) according to manufacturer’s instructions for 48 h [11–13 (link)]. After the transfection efficiency was verified by quantitative real-time PCR (qRT-PCR), the cells were collected and used for further analyses.

Yi C., Gu T., Li Y, & Zhang Q. (2022). Depression of long non-coding RNA SOX2 overlapping transcript attenuates lipopolysaccharide-induced injury in bronchial epithelial cells via miR-455-3p/phosphatase and tensin homolog axis and phosphatidylinositol 3-kinase/protein kinase B pathway. Bioengineered, 13(5), 13643-13653.

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Culturing and Transfecting Human VSMCs

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Human VSMCs were obtained from the Cell Center of the Chinese Academy of Medical Sciences (Shanghai, China), cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, HyClone), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma, St. Louis, MO, USA) at 37°C in 5% CO₂. The cells in the logarithmic growth phase were used for follow-up experiments.
We purchased pcDNA3.1-control, pcDNA3.1-FGF1, miRNA mimics control (mimics-NC), miRNA inhibitors control (inhibitors-NC), miR-188-3p mimics, and miR-188-3p inhibitors from GenePharma Co., Ltd. (Shanghai, China). VSMCs were harvested and seeded into a 24-well plate at a density of 3×10⁵ cells/well, and cell transfection was performed after 24 h of culture. VSMCs were transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) conforming to the protocols.

Mi S., Wang P, & Lin L. (2020). miR-188-3p Inhibits Vascular Smooth Muscle Cell Proliferation and Migration by Targeting Fibroblast Growth Factor 1 (FGF1). Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e924394-1-e924394-12.

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Transfecting Cells with mGluR4 cDNA

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pcDNA3.1 mGluR4 cDNA and pcDNA3.1 control were synthesized by GenePharma Co. (China). They were transfected into BMDC using Effectene Transfection reagent (QIAGEN GmbH, Germany) according to manufacturer instructions. Transfected cells were incubated at 37°C in a 5% CO₂ incubator for 24 h. Total RNA and protein were harvested separately and stored at −80°C until use.

Zhao G., Zhou W., Liu Y., Wang Y., Li Z, & Song Z. (2020). Critical role of metabotropic glutamate receptor 4 in bone marrow-derived dendritic cells in the Th17 cell differentiation and the melanogenesis of B16 cells. Brazilian Journal of Medical and Biological Research, 53(4), e9282.

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Investigating miR-488 and Runx2 in BMSCs

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The RNA oligoribonucleotides [miR-488 mimics (5′-GGGTCTATTACCGTGAGAGTT), miR-488 inhibitor (TTGAGAGTGCCATTATCTGGG-3′), mimics control (5′-TACGTCCAAGGTCGGGCAGGAAGA-3′), inhibitor control (5′-UCCUCCGAACGUGUCACGUTT-3′), pcDNA 3.1-Runx2 and pcDNA 3.1-control] used in the present study were synthesized by Shanghai GenePharma Co., Ltd. Prior to transfection, BMSCs were isolated and seeded (2×10⁶ cells/l) into 6-well plates and grown until they were 60–80% confluent. The cells were then transfected with 100 nM miR-488 mimics, miR-488 inhibitor, mimics control, inhibitor control, pcDNA 3.1-Runx2 or pcDNA 3.1-control for 6 h, using Lipofectamine^® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). The cells were then digested with 0.025% trypsin for 24 h and collected for further analyses, including RTq-PCR, western blotting, a luciferase reporter assay and immunocytochemistry.

Huang Y., Hou Q., Su H., Chen D., Luo Y, & Jiang T. (2019). miR-488 negatively regulates osteogenic differentiation of bone marrow mesenchymal stem cells induced by psoralen by targeting Runx2. Molecular Medicine Reports, 20(4), 3746-3754.

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