Log In Sign up
The largest database of trusted experimental protocols

Q exactive orbitrap hrms

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The Q-Exactive Orbitrap HRMS is a high-resolution mass spectrometry instrument designed for accurate mass measurements and sensitive detection of chemical compounds. It utilizes the Orbitrap mass analyzer technology to provide high mass resolving power and mass accuracy. The instrument is capable of performing full-scan and tandem mass spectrometry (MS/MS) experiments.

Automatically generated - may contain errors

Lab products found in correlation

Vanquish uhplc, by Thermo Fisher Scientific (2 mentions) Ultimate 3000 system, by Thermo Fisher Scientific (1 mentions) Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Hypersil gold c18 column, by Thermo Fisher Scientific (1 mentions) Dionex ultimate 3000 uhplc system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS UPLC-Q Exactive Orbitrap-HRMS system Q-Exactive GC Orbitrap HRMS detector Q Exactive HRMS Q-Orbitrap HRMS Q Exactive Orbitrap Orbitrap Exactive Q Exactive

6 protocols using q exactive orbitrap hrms

1

Targeted Metabolite Confirmation in Placenta Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Targeted chemical confirmation was performed via LCMS using a Thermo Vanquish UHPLC coupled to a Thermo Q-Exactive Orbitrap HRMS. Two confirmation standard mixtures were prepared in DMSO as per standard protocols for mixture preparation: (1) a mixture of priority chemicals (n = 31) that were available within the existing ToxCast chemical screening library (each at 0.625 mM); and (2) a mixture of priority chemicals (n = 16) that required procurement outside of the ToxCast library (each at 1.33 mM). Further details surrounding chemical procurement are included in Supplementary Table 2.
A set of placenta sample extracts, which cumulatively contained all features of interest (preeclamptic samples 1, 2, 3, 5, 10, 12, 14, 15, 18), were run alongside standard mixtures for retention time and mass spectral data alignment with non-targeted analysis data for each targeted chemical. Placenta sample extracts and confirmation standard mixtures were analyzed in MS1 and MS1/MS2 hybrid mode (full scan with parallel reaction monitoring). MS acquisition details can be found in the Supplementary Methods Section 2. Confirmation of chemical identity was established via matching of monoisotopic mass (within 5 ppm), RT (within 0.2 min), and a minimum of one fragment ion (within 0.001 Da) between features observed in placenta samples and the prepared standard mixtures.
Chao A., Grossman J., Carberry C., Lai Y., Williams A.J., Minucci J.M., Purucker S.T., Szilagyi J., Lu K., Boggess K., Fry R.C., Sobus J.R, & Rager J.E. (2022). Integrative exposomic, transcriptomic, epigenomic analyses of human placental samples links understudied chemicals to preeclampsia. Environment international, 167, 107385.
+ Open protocol
+ Expand
2

Targeted Metabolite Confirmation in Placenta Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Targeted chemical confirmation was performed via LCMS using a Thermo Vanquish UHPLC coupled to a Thermo Q-Exactive Orbitrap HRMS. Two confirmation standard mixtures were prepared in DMSO as per standard protocols for mixture preparation: (1) a mixture of priority chemicals (n = 31) that were available within the existing ToxCast chemical screening library (each at 0.625 mM); and (2) a mixture of priority chemicals (n = 16) that required procurement outside of the ToxCast library (each at 1.33 mM). Further details surrounding chemical procurement are included in Supplementary Table 2.
A set of placenta sample extracts, which cumulatively contained all features of interest (preeclamptic samples 1, 2, 3, 5, 10, 12, 14, 15, 18), were run alongside standard mixtures for retention time and mass spectral data alignment with non-targeted analysis data for each targeted chemical. Placenta sample extracts and confirmation standard mixtures were analyzed in MS1 and MS1/MS2 hybrid mode (full scan with parallel reaction monitoring). MS acquisition details can be found in the Supplementary Methods Section 2. Confirmation of chemical identity was established via matching of monoisotopic mass (within 5 ppm), RT (within 0.2 min), and a minimum of one fragment ion (within 0.001 Da) between features observed in placenta samples and the prepared standard mixtures.
Chao A., Grossman J., Carberry C., Lai Y., Williams A.J., Minucci J.M., Purucker S.T., Szilagyi J., Lu K., Boggess K., Fry R.C., Sobus J.R, & Rager J.E. (2022). Integrative exposomic, transcriptomic, epigenomic analyses of human placental samples links understudied chemicals to preeclampsia. Environment international, 167, 107385.
+ Open protocol
+ Expand
3

UHPLC-HRMS Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
UHPLC analysis was performed using an Ultimate 3000 system (Dionex, Sunnyvale, CA, USA), which is equipped with an online vacuum degasser, a quaternary pump, an autosampler, and a thermostated column compartment. An ACQUITY UPLC HSS T3, 2.1 mm × 100 mm, 1.7 μm (Waters, Milford, MA, USA) was used for chromatographic separation at 40 °C. For separation, gradient elution using aqueous formic acid 0.1% (v/v) was done for mobile phase A and acetonitrile for phase B at a flow rate of 0.3 mL/min. The following gradient was applied: 0–1 min, 0% B; 1–10 min, 0%→100% B and 10–10.1 min, 0% B. The injection volume was 2 μL, and the injection temperature was set at 15 °C.
High-Resolution Mass spectrometry was performed with a Q-Exactive Orbitrap HRMS (Thermo Fisher, Waltham, MA, USA) using a heated electrospray ionization source (HESI) for the ionization of the target compounds in the negative mode. The operating parameters were as follows: spray voltage, 3.70 KV; capillary temperature, 320 °C; sheath gas pressure, 30 psi; auxiliary gas pressure, 10 arb; auxiliary gas heater temp, 300 °C; scan modes, full MS scan (resolution 70,000); and scan range, m/z 100–1500. The data were processed using the Thermo Xcalibur 3.0 software (Thermo Finnigan, San Jose, CA, USA).
Du Z., Li J., Zhang X., Pei J, & Huang L. (2018). An Integrated LC-MS-Based Strategy for the Quality Assessment and Discrimination of Three Panax Species. Molecules, 23(11), 2988.
+ Open protocol
+ Expand
4

UHPLC-MS/MS Metabolite Profiling Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The UHPLC-MS/MS measurement was performed using Ultimate 3000 liquid chromatography coupled with Q-Exactive Orbitrap HRMS (Thermo Fisher Scientific, Waltham, MA, USA) based on the previously described method [26 (link)]. A chromatographic separation was performed on a Thermo Hypersil GOLD C18 column (100 mm × 2.1 mm, 1.9 µm) maintained at 40 °C, with a flow rate of 0.4 mL/min. The samples were kept under 4 °C and injection volume was 5 µL. The gradient elution was composed of mobile phase A (0.1% formic acid in water) and mobile phase B (0.1% formic acid in acetonitrile) and performed for 22 min, as shown in Supplementary Table S1. The Q-Exactive Orbitrap HRMS was equipped with heated electrospray ionization (HESI) source using the following source parameters: sheath gas flow—55 arb (arbitrary units); aux gas flow—10 arb; spray voltage—+4.0/−3.5 KV; capillary temperature—350 °C; S-lens RF level—55; aux gas heater temperature—300 °C. For MS, resolution was set as 70,000 and Scan range was 70 to 1000. For MS/MS, data-dependent acquisition (DDA) mode was used, and the top 10 precursors were fragmented by 15, 30, and 45 collision energy values.
Shiri T.J., Viau C., Gu X., Xu L., Lu Y, & Xia J. (2023). The Native Microbiome Member Chryseobacterium sp. CHNTR56 MYb120 Induces Trehalose Production via a Shift in Central Carbon Metabolism during Early Life in C. elegans. Metabolites, 13(8), 953.
+ Open protocol
+ Expand
5

Embryo Lipidomics and Metabolomics Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The embryos
were prepared through
an in-plate extraction method described previously.14 (link) In short, 120 μL of a MeOH:chloroform (80:20) mixture
containing an IS was added to each well along with mixed-size stainless
steel beads. A silicone lid with a polytetrafluorethylene surface
layer was glued onto the plates prior to homogenization, sonication,
and centrifugation. Following extraction, the samples were subjected
to both targeted lipidomics and nontarget metabolomics analysis.13 (link) For the lipidomics analysis, plates were fitted
directly into the autosampler of the same UHPLC–MS/MS system
used for dose characterization (see previous section). After the flow-injection
lipidomics analysis, the plate was moved to the autosampler of another
Ultimate3000 UHPLC fitted with a hydrophilic interaction liquid chromatography
(HILIC) column (BEH amide; Waters, USA) connected to a Q Exactive
Orbitrap HRMS (Thermo, USA) via an electrospray ionization source.
The instrument was operated in positive mode utilizing nontargeted
full scan acquisition with data-dependent MS2 analysis of the three
highest-intensity features.
Ribbenstedt A., Posselt M, & Benskin J.P. (2022). Toxicometabolomics and Biotransformation Product Elucidation in Single Zebrafish Embryos Exposed to Carbamazepine from Environmentally-Relevant to Morphologically Altering Doses. Chemical Research in Toxicology, 35(3), 431-439.
+ Open protocol
+ Expand
6

UHPLC-HRMS Analysis of Metabolites

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were analyzed with a Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific, San Jose, CA, United States) linked to a Q-Exactive Orbitrap HRMS (Thermo Fisher Scientific, San Jose, CA, United States). Chromatographic separation was performed on a Hypersil Gold UPLC C18 (2.1 mm × 150 mm, 1.9 μm) reversed phased column (Thermo Fisher Scientific, San Jose, CA, United States). The ionization was performed using heated electrospray (ESI) in both positive and negative modes. Chromatographic and mass spectrometry conditions and parameters are described in the previous work of our group (Stavropoulou et al., 2021 (link)). To monitor the stability and repeatability of the instrumental procedure, a quality control (QC) sample was analyzed three times before and at the end of the sequence, and after every six experimental samples. The QC sample was prepared as a pool of all study samples.
Kasiotis K.M., Baira E., Iosifidou S., Bergele K., Manea-Karga E., Theologidis I., Barmpouni T., Tsipi D, & Machera K. (2022). Characterization of Ikaria Heather Honey by Untargeted Ultrahigh-Performance Liquid Chromatography-High Resolution Mass Spectrometry Metabolomics and Melissopalynological Analysis. Frontiers in Chemistry, 10, 924881.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!