Three hundred treated cells suspended in 750 μl medium were plated in per well of 24-well plate, then 750 μl of 2.7% methylcellulose (Sigma, USA) was added in and stirred well with the medium. After culturing for 7–10 days, the number of colonies was counted with an inverted micro-scope (Nikon C-HGF1, Japan). Each group included four counterparts and was repeated for three times.
C hgf1
Manufactured by Nikon
Sourced in Japan
The C-HGF1 is a Nikon microscope accessory designed for laboratory use. It provides high-quality illumination for microscopic samples. The device features a compact and durable construction, ensuring reliable performance in various lab environments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using c hgf1
1
Methylcellulose Colony Formation Assay
2
Fluorescence Imaging of Stone Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stone cells were observed using the method specified by Liu et al. [2] (link). Specifically, fruits were fixed with FAA (formaldehyde, acetic acid, ethanol) for 3 h, soaked in glycerol and sectioned. Then the sections were observed under a Ni-U fluorescence microscope with C-HGF1 fluorescence emission source and Fi3 camera (Nikon, Tokoy, Japan). Finally, blue fluorescence of lignin in the stone cells was observed under the excitation wavelength of 380 nm of purple light.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!