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Ramucirumab

Manufactured by Selleck Chemicals
Sourced in United States

Ramucirumab is a laboratory equipment product used for research purposes. It is a monoclonal antibody that targets the vascular endothelial growth factor receptor 2 (VEGFR-2). Ramucirumab is used in scientific research to study angiogenesis and its role in various biological processes.

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2 protocols using ramucirumab

1

Apoptosis Detection Using Ramucirumab

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Ramucirumab, paclitaxel, and tepotinib were obtained from Selleck Chemicals (Houston, TX, USA). The annexin V-APC/propidium iodide (PI) apoptosis detection kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA).
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2

Cell Line Maintenance and Inhibitor Compounds

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HCC827 and PC-9 cells were purchased from the American Type Culture Collection and RIKEN Cell Bank, respectively. H1975 cells containing EGFR-L858R and T790M double mutation were provided by Dr. Yoshitaka Sekido (Aichi Cancer Center Research Institute) and Dr. John D. Minna. The cells were maintained in RPMI 1640 medium with 10% fetal bovine serum and 1% penicillin and streptomycin in 5% CO2 at 37°C. All cells were passaged for less than 3 months and tested for mycoplasma using a MycoAlert Mycoplasma Detection Kit (Lonza). Erlotinib (first-generation EGFR TKI), osimertinib (third-generation EGFR TKI), ONO-7475 (AXL inhibitor), and ramucirumab (human anti–vascular epithelial growth factor receptor 2 antibody) were obtained from Selleck Chemicals.
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