Ammonium formate, hematoxylin, and eosin were purchased from Sigma Aldrich (St. Louis, MO, USA). 1,5-diaminonapthaline (DAN) and 2,5-dihydroxyacetophenone (DHA) were also purchased from Sigma Aldrich (St. Louis, MO, USA) then purified by recrystallization. Indium tin oxide (ITO) coated slides were purchased from Delta Technologies, Limited (Loveland, CO, USA). All solvents (methanol, ethanol, acetonitrile, acetic acid, chloroform, and xylenes) and optimal cutting temperature compound were purchased from Fisher Scientific (Kalamazoo, MI, USA).
2 5 dihydroxyacetophenone
Manufactured by Merck Group
Sourced in United States
2,5-dihydroxyacetophenone is a chemical compound that serves as a laboratory reagent. It is a white crystalline solid with the molecular formula C₈H₈O₃. The compound functions as a precursor for various organic synthesis reactions, but no further details on its intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (4 mentions)
Ethanol, by Thermo Fisher Scientific (3 mentions)
Ammonium formate, by Merck Group (3 mentions)
2 5 dihydroxybenzoic acid, by Merck Group (3 mentions)
Hematoxylin, by Merck Group (3 mentions)
Chloroform, by Thermo Fisher Scientific (2 mentions)
Eosin y, by Avantor (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (2 mentions)
Sinapic acid, by Merck Group (2 mentions)
Acetone, by Thermo Fisher Scientific (2 mentions)
1 5 diaminonaphthalene, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (2 mentions)
Hplc grade chloroform, by Merck Group (2 mentions)
Optimal cutting temperature compound, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Haematoxylin, by Avantor (1 mentions)
Optima lc ms grade, by Thermo Fisher Scientific (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
9 protocols using 2 5 dihydroxyacetophenone
1
Histochemical Staining Protocol
2
Mass Spectrometry Sample Preparation
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Isopropanol (LC-MS grade), ethanol (LC-MS grade), 2,5-dihydroxybenzoic acid (DHB, ≥ 99.9% purity), 2,5-dihydroxyacetophenone (DHA) and water (LC-MS grade) were purchased from Sigma Aldrich (Zwijndrecht, The Netherlands) and used without further purification. Haematoxylin (Merck, Darmstadt, Germany) and eosin Y (J.T. Baker, Center Valley, PA, USA) were used under standard laboratory protocols. Indium tin oxide (ITO)-coated glass slides were purchased from Delta Technologies (Loveland, USA).
3
Lipid and MALDI-MS Sample Preparation
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methanol (LC-MS grade), acetonitrile (Optima®), water (Optima®), and isopropanol (Optima® LC-MS grade) for lipid LC-MS were obtained from Fisher Scientific, Scorseby, Victoria, Australia. Ammonium acetate for lipid LC-MS was obtained from Sigma-Aldrich, North Ryde, New South Wales, Australia. For MALDI-MS-related sample preparation and analysis, sodium acetate (≥99%), 2,5-dihydroxyacetophenone (≥99.5%, Ultrapure), methanol (LC-MS grade), and chloroform (HPLC grade) were obtained from Sigma-Aldrich, Zwijndrecht, The Netherlands.
4
Metabolite Profiling of Edible Burdock Roots
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Fresh A. lappa roots of Baiji variety (Chinese name) and Yanagawa-riso variety were purchased from Linyi city (Shandong, China).
α-Cyano-4-hydroxycinnamic acid (CHCA, pure 99.0%), sinapic acid (SA, pure 99.0%), 2′,5′-dihydroxyacetophenone (DHAP, pure 97%), 9-aminoacridine (9-AA, pure 99.5%), N-(1-naphthyl)-ethylenediamine dihydrochloride (NEDC, pure 98.0%), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (ST. Louis, MO, USA). L-arginine, proline, phenylalanine and 2,5-dihydroxybenzoic (DHB, pure 99.0%) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Caffeic acid, quercetin, chlorogenic acid, ursolic acid, 1,5-dicaffeoylquinic acid, and 1,3,5-tricaffeoylquinic acid (pure 98.0%) were provided by the Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). HPLC-grade acetonitrile (ACN) and methanol (MeOH) were supplied by Merck (Darmstadt, Germany). Optically transparent indium-tin-oxide (ITO) coated glass slides were obtained from Huanan Xiangcheng Technology Co., Ltd. (Shenzhen, China). The optimum cutting temperature (OCT) compound was supplied by SAKURA (Torrance, CA, USA).
α-Cyano-4-hydroxycinnamic acid (CHCA, pure 99.0%), sinapic acid (SA, pure 99.0%), 2′,5′-dihydroxyacetophenone (DHAP, pure 97%), 9-aminoacridine (9-AA, pure 99.5%), N-(1-naphthyl)-ethylenediamine dihydrochloride (NEDC, pure 98.0%), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (ST. Louis, MO, USA). L-arginine, proline, phenylalanine and 2,5-dihydroxybenzoic (DHB, pure 99.0%) were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Caffeic acid, quercetin, chlorogenic acid, ursolic acid, 1,5-dicaffeoylquinic acid, and 1,3,5-tricaffeoylquinic acid (pure 98.0%) were provided by the Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). HPLC-grade acetonitrile (ACN) and methanol (MeOH) were supplied by Merck (Darmstadt, Germany). Optically transparent indium-tin-oxide (ITO) coated glass slides were obtained from Huanan Xiangcheng Technology Co., Ltd. (Shenzhen, China). The optimum cutting temperature (OCT) compound was supplied by SAKURA (Torrance, CA, USA).
5
Lipid Extraction and Analysis Protocol
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HPLC grade water and acetonitrile (ACN) were purchased from (VWR International-UK). Acetone was purchased from (Fisher Scientific, Loughborough, UK). HPLC grade methanol (MeOH, ≥99.9%), HPLC grade chloroform (CHCl3, ≥99.9%), 5-chloro-2-mercaptobenzothiazole (5C2M), 2-mercaptobenzothiazole, 6-aza-2-thiothymine, 2-(4-hydroxyphenylazo) benzoic acid, 2,5-dihydroxy benzoic acid (DHB), super-DHB, sinapic acid, 2,4,6-trihydroxy acetophenone monohydrate, 2,5-dihydroxy acetophenone, α-cyano-4-hydroxycinnamic acid, picolinic acid, and 9-aminoacridine hemihydrate were purchased from (Sigma Aldrich, Gillingham, UK). Porcine Gb3 standard and N-heptadecanoyl ceramide trihexoside (C17:0) internal standard were purchased from Matreya (Pleasant Gap, PA, USA).
6
Lipid Analysis by Mass Spectrometry
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2,5-Dihydroxyacetophenone (DHA, 97% purity),
sodium acetate (anhydrous, >99%), and chloroform (≥99%)
were
purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) and used
without further purification. Methanol (ULC-MS grade), water (LC-MS
grade), ethanol (LC-MS grade), and xylene (AR grade) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hematoxylin and Entellan
were purchased from Merck (Darmstadt, Germany) and eosin Y from J.T.
Baker (Center Valley). Indium tin oxide (ITO)-coated glass slides
were purchased from Delta Technologies (Loveland). Lipid standards
(PC 18:0/18:1n-9 and PC 16:0/18:1n-9) were purchased from Avanti Polar Lipids (Alabaster).
sodium acetate (anhydrous, >99%), and chloroform (≥99%)
were
purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands) and used
without further purification. Methanol (ULC-MS grade), water (LC-MS
grade), ethanol (LC-MS grade), and xylene (AR grade) were purchased
from Biosolve (Valkenswaard, The Netherlands). Hematoxylin and Entellan
were purchased from Merck (Darmstadt, Germany) and eosin Y from J.T.
Baker (Center Valley). Indium tin oxide (ITO)-coated glass slides
were purchased from Delta Technologies (Loveland). Lipid standards
(PC 18:0/18:1n-9 and PC 16:0/18:1n-9) were purchased from Avanti Polar Lipids (Alabaster).
7
Tissue Preparation and Chemical Analysis
8
Synthesis and Characterization of Nanoparticles
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2′,5′-Dihydroxyacetophenone (DHA), 1′,5′-Diaminonaphthalene (DAN), ammonium formate, ammonium acetate, and poly-l -lysine solution were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). HPLC-grade acetonitrile, methanol, and gelatin powder were purchased from Fisher Scientific (Pittsburgh, PA, USA). Carboxymehtylcellulose (CMC) was purchased from PM MP Biomedicals (Salon, OH). Tryptic soy agar (TSA) and tryptic soy broth (TSB) were purchased from Becton Dickinson (Franklin Lakes, NJ) and all lab plasticware was from USA Scientific (Ocala, FL) or Corning (Corning, NY). Whatman Nucleopore polycarbonate filter discs (0.1 um pore size) were acquired from Millipore Sigma (Burlington, MA).
9
Lipid Extraction and Characterization
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Materials: 1,5-diaminonaphthalene (DAN) and 2',5'dihydroxyacetophenone (DHA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade acetonitrile, methanol, ethanol, chloroform, and tetrahydrofuran (THF) were purchased from Fisher Scientific (Pittsburgh, PA, USA). Lipid standards were purchased from Avanti Polar Lipids (Alabaster, AL, US).
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