Mouse anti grp78

Manufactured by R&D Systems

Sourced in United States

Mouse anti-GRP78 is a primary antibody that recognizes the GRP78 (Glucose-Regulated Protein 78) protein, which is a member of the heat shock protein 70 (HSP70) family. GRP78 is a molecular chaperone that plays a crucial role in the endoplasmic reticulum (ER) stress response. This antibody can be used to detect and study the expression and localization of GRP78 in various experimental settings.

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Lab products found in correlation

Rabbit anti hsp60, by Abcam (1 mentions) Rabbit anti phospho eif2a, by Cell Signaling Technology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Rabbit anti caspase 3, by Abcam (1 mentions) Mouse anti chop antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti guinea pig, by Thermo Fisher Scientific (1 mentions) Rabbit anti p erk, by Abcam (1 mentions) Mouse anti his, by Abcam (1 mentions) Alexa fluor 568 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Rabbit anti grp78, by Abcam (1 mentions) Mouse anti v5 antibody, by Immunoway (1 mentions)

2 protocols using mouse anti grp78

Comprehensive Western Blotting Protocol

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Western blotting was conducted according to standard protocols. The antibodies used in this study were as follows: rabbit anti-WWOX (a kind gift of Dr. M Aldaz, MD Anderson Cancer Centre, Houston, TX, USA) and ab137726 antibody (abcam), rabbit anti-β-actin (no. 4970; Cell Signalling Technology, Danvers, MA, USA), rabbit anti-Hsp60 (abcam; ab46798), rabbit anti-cleaved (Asp175) caspase-3 (R&D Systems, Minneapolis, MN, USA; 269518), mouse caspase-2 (no. 2224; Cell Signalling Technology), rabbit caspase-7 (no. 9492; Cell Signalling Technology), rabbit anti-phospho-eIF2A (Cell Signalling Technology; 9721), mouse anti-GRP78 (474421; R&D Systems), mouse anti-JNK1/JNK2 252323, MAB2076 and rabbit AF1205 (phospho-JNK) (R&D Systems), rabbit anti-phospho (T183/Y185)-JNK (AF1205), rabbit IRE-1 (ab48187 and ab37073; abcam) and PERK (no. 3192; Cell Signalling Technology).

Janczar S., Nautiyal J., Xiao Y., Curry E., Sun M., Zanini E., Paige A.J, & Gabra H. (2017). WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response. Cell Death & Disease, 8(7), e2955-.

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MERS-CoV and SARS-CoV-2 Protein Detection

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MERS-CoV N was detected with the in-house guinea pig anti–MERS-CoV N serum. MERS-CoV S was detected with the in-house mouse anti–MERS-CoV S immune serum. MERS-CoV M and SARS-CoV-2 M were detected with in-house mouse anti–MERS-CoV M and mouse anti–SARS-CoV-2 M immune serum, respectively. SARS-CoV M was detected with a rabbit anti–SARS-CoV M antibody from Rockland. Primary antibodies including rabbit anti–caspase-3, rabbit anti-PERK, rabbit anti-GRP78, and mouse anti-His were from Abcam. The mouse anti-CHOP antibody was from Cell Signaling Technology. The mouse anti-GRP78 was from R&D Systems. The mouse anti–β-actin was from Sigma-Aldrich. The V5-tagged proteins were detected with a mouse anti-V5 antibody from Immunoway. Secondary antibodies including Alexa Fluor 488 goat anti–guinea pig, Alexa Fluor 488 goat anti-mouse, and Alexa Fluor 568 donkey anti-rabbit were from Thermo Fisher Scientific. The goat anti-mouse horseradish peroxidase (HRP), goat anti-rabbit HRP, and goat anti–guinea pig antibodies from Thermo Fisher Scientific were used for Western blots and immunohistochemistry staining.

Chu H., Shuai H., Hou Y., Zhang X., Wen L., Huang X., Hu B., Yang D., Wang Y., Yoon C., Wong B.H., Li C., Zhao X., Poon V.K., Cai J.P., Wong K.K., Yeung M.L., Zhou J., Au-Yeung R.K., Yuan S., Jin D.Y., Kok K.H., Perlman S., Chan J.F, & Yuen K.Y. (2021). Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity. Science Advances, 7(25), eabf8577.

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