The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery); WRN (4666S,1:1000 WB,Cell Signaling); BLM (A300-110A,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2000 WB, Bethyl Lab); Flag (F3165, 1:2000, Sigma); Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); γH2AX (05-636, 1:500 IF, Millipore); Claspin (2800S, 1:1000 WB, Cell Signaling); c-MYC (SC-40, 1:200 WB, Santa Cruz); Phospho-Chk1-Ser345 (2348, 1:1000 WB, Cell Signaling); CHK1 (ab32531, 1:1000 WB, Abcam); GAPDH (60004-1-1g, 1:5000 WB, Proteintech); Tubulin (66240-1-1g, 1:5000 WB, Proteintech); ACTIN (66009-1-1g, 1:3000 WB, Proteintech). The secondary antibodies conjugated to horseradish peroxidase for Western blot and the secondary antibodies anti-mouse, -goat or -rabbit Alexa fluor 488 or 594 for immunofluorescence staining were purchased from Jackson ImmunoResearch Laboratories. siRNAs were synthesized by GenePharma.
Blm a300 110a
Manufactured by Fortis Life Sciences
Sourced in United States
The BLM (A300-110A) is a laboratory equipment product designed for basic life science applications. It serves as a core functional component within the research and development workflow. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Usp28 17707 1 ap, by Proteintech (2 mentions)
Recql1 a300 450a, by Fortis Life Sciences (2 mentions)
Wrn 4666s, by Cell Signaling Technology (2 mentions)
Flag f3165, by Merck Group (1 mentions)
C myc sc 40, by Santa Cruz Biotechnology (1 mentions)
γh2ax 05 636, by Merck Group (1 mentions)
Ub sc 8017, by Santa Cruz Biotechnology (1 mentions)
Phospho chk1 ser345 2348, by Cell Signaling Technology (1 mentions)
Gapdh 60004 1 1g, by Proteintech (1 mentions)
2 protocols using blm a300 110a
1
Antibody Characterization for Helicase Enzymes
2
Western Blot Antibodies for DNA Repair
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech, Wuhan, China); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery, TX, USA); WRN ( 4666S,1:1000 WB ,Cell Signaling, Beverly, MA, USA); BLM (A300-110A ,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP ,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2,000 WB, Bethyl Lab); Flag (F3165,
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