GC-MS analysis was performed as previously described (34 (link), 35 (link)) using a QP2010 gas chromatography-mass spectrometer (GC-2010 coupled with a GC-MS QP-2010 Ultra) equipped with an autosampler (AOC-20i+s) from Shimadzu (Tokyo, Japan), using Rtx-5 ms column (30 m length × 0.25 mm inner diameter × 0.25 µm film thickness: Restek, Bellefonte, PA, USA). Helium (99.9% purity) was used as the carrier gas with a column flow rate of 1 ml/min. The column temperature regime was initially adjusted at 35°C for 2 min, followed by an increase in the rate of 10°C/min to reach 250°C. The temperature was then increased by 20°C/min until reaching 320°C and kept for 23 min. The injection volume was 1 µl and the injection temperature was 250°C using splitless injection mode. The mass spectrometer was operated in electron compact mode with electron energy of 70 eV. Both the ion source temperature and the interface temperature were set at 240°C and 250°C, respectively. The MS mode was set on scan mode starting from 35 to 450 m/z with a scan speed of 1,428. Data collection and analysis were performed using MSD Enhanced Chemstation software (Shimadzu). Product spectra were identified by comparison of the measured fragmentation patterns to those found in the NIST 08 Mass Spectral Library.
Msd enhanced chemstation software
Manufactured by Shimadzu
The MSD Enhanced Chemstation software is a comprehensive data analysis and instrument control software for Shimadzu mass spectrometers. It provides advanced features for data acquisition, processing, and reporting.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using msd enhanced chemstation software
1
GC-MS Analysis of Compound Identification
2
GC-MS Analysis of Metabolite Extracts
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GC-MS analysis was performed as described before [18 (link)]. The metabolite extracts were derivatized by adding 50 μL of N-trimethylsilyl-N-methyl trifluoroacetamide and trimethylchlorosilane (MSTFA + 1% TMS) followed by incubation at 50°C for 30 min prior to GC-MS analysis. The derivatized samples were injected into QP2010 gas chromatography-mass spectrometer (GC-2010 coupled with a GC-MS QP-2010 Ultra) equipped with an auto-sampler (AOC-20i+s) from Shimadzu (Tokyo, Japan), using Rtx-5ms column (30 m length × 0.25 mm inner diameter × 0.25 μm film thickness; Restek, Bellefonte, PA). Data collection and analysis were performed using MSD Enhanced Chemstation software (Shimadzu). Product spectra were identified by comparison of the measured fragmentation patterns to those found in the NIST 08 Mass Spectral Library.
3
GC-MS Analysis of Organic Compounds
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GC–MS analysis was performed using a QP2010 gas chromatography-mass spectrometer (GC-2010 coupled with a GC–MS QP-2010 Ultra) equipped with an auto-sampler (AOC-20i+s) from Shimadzu (Tokyo, Japan), using Rtx-5ms column (30 m length × 0.25 mm inner diameter × 0.25 µm film thickness; Restek, Bellefonte, PA, USA). Helium (99.9% purity) was used as the carrier gas with the column flow rate of 1 mL/min. The column temperature regime was initially adjusted at 35 °C for 2 min; followed by an increase in a rate of 10 °C/min to reach 250 °C. The temperature was then increased by 20 °C/min until reaching 320 °C and kept for 23 min. The injection volume and injection temperature were 1 µL and 250 °C using splitless injection mode, respectively. The mass spectrometer operated in electron compact mode with electron energy of 70 eV. Both the ion source temperature and the interface temperature were set at 240 °C and 250 °C, respectively. The MS mode was set on scan mode starting from 35 to 450 m/z with a scan speed of 1428. Data collection and analysis were performed using MSD Enhanced Chemstation software (Shimadzu). Product spectra were identified by comparison of the measured fragmentation patterns to those found in the NIST 08 Mass Spectral Library.
4
GC-MS Analysis of Organic Compounds
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G.C.–M.S. analysis was performed using a QP2010 gas chromatography-mass spectrometer (GC-2010 coupled with a G.C.–MS QP-2010 Ultra) equipped with an auto-sampler (AOC-20i+s) from Shimadzu (Tokyo, Japan), using Rtx-5ms column (30 m length × 0.25 mm inner diameter × 0.25 μm film thickness; Restek, Bellefonte, PA, USA). Helium (99.9% purity) was used as the carrier gas with a 1 mL/min column flow rate. The column temperature regime was initially adjusted at 35°C for 2 min, followed by an increase in a rate of 10°C/min to reach 250°C. The temperature was then increased by 20°C/min until reaching 320°C and kept for 23 min. The injection volume and injection temperatures were 1 μL and 250°C using splitless injection mode, respectively. The mass spectrometer operated in electron impact mode with electron energy of 70 eV. The ion source temperature and the interface temperature were set at 240°C and 250°C, respectively. The MS mode was set on scan mode starting from 50–650 m/z with a scan speed of 1428. Data collection and analysis were performed using MSD Enhanced Chemstation software (Shimadzu). G.C. total ion chromatograms (TIC) and fragmentation patterns of the compound were identified using the NIST/EPA/NIH Mass Spectral Library (NIST 14) (S1 File ). The run time for each sample was 43.67 min [31 (link)].
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