Peroxidase affinipure goat anti rabbit igg 111 035 003

Reagents were purchased from Sigma-Aldrich or indicated suppliers. Anti-β-actin mouse (sc-47778) and anti-LL-37 mouse monoclonal antibodies (sc-166770) were purchased from Santa Cruz Biotechnology. Anti-cathelicidin rabbit monoclonal antibodies (ab207758) were purchased from Abcam. Peroxidase-AffiniPure goat anti-rabbit IgG (111-035-003), peroxidase-AffiniPure goat anti-mouse IgG (115-035-003), and fluorescein (FITC)-AffiniPure donkey anti-mouse IgG (715-095-151) were purchased from Jackson ImmunoResearch Laboratories. The SARS-CoV-2 spike protein (Z03481) was purchased from GenScript. Human alpha thrombin (HT 1002a) and human factor Xa (HFXa 1011) were purchased from Enzyme Research Laboratories.

The following antibodies were used: anti-OTUB1 (ab175200), anti-OTUB1 (ab198214, for IHC), anti-Smurf1 (ab57573), anti-RunX2 (ab192256), and anti-OSX (ab209484), purchased from Abcam. Anti-OTUB2 antibody (GTX83953) was purchased from GeneTex. Anti-FGFR1 (9740), anti-FGFR2 (23328), anti-FGFR3 (4574), anti-AKT (4691), anti-p-Ser473-AKT (4060), anti-p-Ser308-AKT (13038), anti-ERK1/2 (4695), and anti-p-Thr202/Tyr204-ERK1/2 (4370) antibodies were purchased from Cell Signaling Technology. Anti-GAPDH (sc-365062) and anti-GST (sc-374171) antibodies were purchased from Santa Cruz Biotechnology. Anti-Myc (M047-3), anti-Flag (M185-3), anti-HA (M180-3), and anti-Multi Ubiquitin (D058-3) antibodies were purchased from MBL. Anti-β-actin (AC026) and anti-COL1A1 (A16891) antibodies were purchased from Abclonal. Peroxidase-AffiniPure goat anti-rabbit IgG (111-035-003) and Peroxidase-AffiniPure goat anti-mouse IgG (115-035-003) were purchased from Jackson.
Full-length OTUB1 WT, D88A, C91S, H265A, ASA mutants, and Flag-HECT E3s were cloned into the pFlag-CMV-2 vector. Full-length FGFR2 was cloned into the pEF6/Myc-His vector.