At the end of the experiment, the mice were left unfed overnight. After exposure to a cold environment (4°C) for 30 min, the mice were lightly anesthetized with isoflurane and injected with 18F-fluorodeoxyglucose (F-FDG) (500 mCi) via the tail vein. They were then subjected to positron emission tomography-computed tomography (PET-CT) imaging 60 min after the radiotracer injection, using the Siemens Inveon Dedicated PET System and the Inveon Multimodality System (CT/SPECT) (Siemens Preclinical Solutions, Knoxville, TN, USA) at the Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences. The Inveon Acquisition Workplace software was used for the scanning process. The PET-CT instrument parameters and data analysis adhered to previously described methods (24 (link)).
Inveon dedicated pet system
Manufactured by Siemens
Sourced in United States
The Inveon Dedicated PET System is a preclinical positron emission tomography (PET) imaging device designed for small animal research. It provides high-resolution PET imaging capabilities for studying small laboratory animals, such as mice and rats. The system is optimized for PET imaging and offers robust performance and reliability for preclinical research applications.
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Lab products found in correlation
4 protocols using inveon dedicated pet system
1
In Vivo PET-CT Imaging of Mice
2
Measuring Myocardial Glucose Uptake with PET
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Three rounds of positron‐emission tomography (PET) scan were carried out at 8 weeks after surgery to evaluate the MGU in vivo with an Inveon dedicated PET system (Siemens, Bonn, Germany). Briefly, the first round was carried out regularly after fluorine‐18 labeled fluorodeoxyglucose (18F‐FDG) injection from the tail vein. To ensure the maximal stimulation of MGU, the second round was carried out after an intravenous glucose load. To fully simulate the postprandial hyperglycemia state, the third round was carried out after an intragastric glucose load instead. To determine the MGU, multiple regions‐of‐interest were drawn manually on at least five representative midventricular transverse planes by visualizing the heart margin as they appeared as bumps under the skin23 . The mean standard uptake value was calculated to identify the radioactivity of the heart. The detailed method and image processing are listed in Appendix S1 .
3
PET Imaging of Mrp1 Knockout Mice
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Four
WT mice and four mrp1 KO mice were used for PET scanning
with [18F]12 . Body temperature was monitored
using a rectal thermometer throughout PET scanning and was maintained
within the normal range using a heating pad system. Scans were performed
with an Inveon Dedicated PET system (Siemens Medical Solutions, Knoxville,
TN, USA), which has a transaxial field of view of 10 cm and an axial
field of view of 12.7 cm. The compound [18F]12 (3.6 MBq; <0.04 nmol) was administered as a 0.2 mL intravenous
bolus injection to each mouse, which was maintained under isoflurane
anesthesia during the scanning periods. Data were acquired by the
animal tomograph for 60 min after injection in 20 frames divided as
follows: 4 × 1 min, 8 × 2 min, and 8 × 5 min frames.
Without attenuation correction, the data were reconstructed using
Fourier rebinning and filtered back projection with a Hanning filter
cutoff at the Nyquist frequency into images with a 128 × 128
matrix size and 3 times zoom, to give a voxel size of 0.26 ×
0.26 × 0.8 mm3. After image reconstruction, volumes
of interest were manually placed on a summed PET image (coronal view
of the brain) and transferred to all of the frames of images to generate
time–radioactivity concentration (Bq/mL) curves for whole brain,
using the ASIPro VM software (CTI Concorde Microsystems, Knoxville,
TN, USA).
WT mice and four mrp1 KO mice were used for PET scanning
with [18F]
using a rectal thermometer throughout PET scanning and was maintained
within the normal range using a heating pad system. Scans were performed
with an Inveon Dedicated PET system (Siemens Medical Solutions, Knoxville,
TN, USA), which has a transaxial field of view of 10 cm and an axial
field of view of 12.7 cm. The compound [18F]
bolus injection to each mouse, which was maintained under isoflurane
anesthesia during the scanning periods. Data were acquired by the
animal tomograph for 60 min after injection in 20 frames divided as
follows: 4 × 1 min, 8 × 2 min, and 8 × 5 min frames.
Without attenuation correction, the data were reconstructed using
Fourier rebinning and filtered back projection with a Hanning filter
cutoff at the Nyquist frequency into images with a 128 × 128
matrix size and 3 times zoom, to give a voxel size of 0.26 ×
0.26 × 0.8 mm3. After image reconstruction, volumes
of interest were manually placed on a summed PET image (coronal view
of the brain) and transferred to all of the frames of images to generate
time–radioactivity concentration (Bq/mL) curves for whole brain,
using the ASIPro VM software (CTI Concorde Microsystems, Knoxville,
TN, USA).
4
Adipose Depot PET Imaging in Mice
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Prior to injection, mice were fasted for about 3 hours and were i.v. injected with 200–300 μCi 18F-FDG. After injection, the 30°C group was kept at 30°C, while the 4°C, –10°C, and –10°C/–20°C groups were kept in cold. After 1 hour, adipose depots were quickly dissected and scanned by the Inveon Dedicated PET System (Siemens).
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