Phenylboronic acid (>97.0% purity), 3-MCPD (>98% purity), 1,3-DCP (>98% purity), and d5 1,3-DCP (>98% purity) were purchased from Sigma-Aldrich (St. Louis, MO), and d5 3-MCPD (>98% purity) was purchased from Canadian isotopes (Point-Claire, Quebec, Canada). Sodium chloride crystals, acetonitrile, and LC-MS Optima water were purchased from Fisher Scientific (Fair Lawn, NJ, USA). For the QuEChERS method, salt extraction mylar pouches (AOAC 2007.1, 6 g of MgSO4 with 1.5 g of sodium acetate) and dSPE pouches (400 mg of PSA, 400 mg of C18) were purchased from United Chemical (Bristol, PA). Additionally, a FAPAS proficiency standard for 1,3-DCP and 3-MCPD in soy sauce was purchased from Sigma-Aldrich (St. Louis, MO). Stock solutions of 3-MCPD and d5 3-MCPD were initially prepared in 20% NaCl in water (PBA method) and 100% ethyl acetate (QuEChERS method) at concentrations of 2 mg/mL and further diluted for recovery and calibration solutions. Additionally, solutions of 1,3-DCP and d5 1,3-DCP were also prepared in ethyl acetate for the QuEChERS method. For the PBA and QuEChERS methods, seven-point calibration curves were prepared that corresponded to 3-MCPD and 1,3-DCP concentrations of 10–2600 μg/kg in soy sauce.
Sodium chloride crystals
Manufactured by Thermo Fisher Scientific
Sourced in United States
Sodium chloride crystals are a common laboratory chemical used for a variety of applications. Sodium chloride, commonly known as table salt, is a crystalline solid compound with the chemical formula NaCl. It is a white, odorless, and water-soluble substance.
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Lab products found in correlation
2 protocols using sodium chloride crystals
1
Quantification of 3-MCPD and 1,3-DCP in Soy Sauce
2
Quantifying Liposomal Peptide Uptake
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To quantify the accumulation of the peptide decorated liposomes inside the cells, flow cytometry was performed after 4-hour incubation of the cells with liposomes. Cells were collected by trypsonization after 4 hours, rinsed 3 times with 1x Dulbecco’s phosphate buffered saline (DPBS) (Formulation: 200mg/L KCl and KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4*7H2O, pH 7–7.6,) (BioWhittaker, Lonza) fixed with 3.7% paraFormaldehyde solution prior to running in flow cytometer (Guava easyCyte 6HT, Luminex). The fixing solution was prepared by mixing 10% formaldehyde (36.5–38% Formaldehyde solution in H2O, Sigma) and 89% DPBS. 1% 1.5M NaCl solution prepared with Sodium Chloride crystals (Fisher Bioreagents) in H2O was added to balance the pH level of the solution.
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