For measuring lifespan, 3 cohorts (replicates) of approximately 80 mated male and 80 mated female flies of each genotype (n=240) were housed separately in 8 oz round bottom polypropylene bottles (Genesee Scientific, San Diego, CA, USA) inverted over 60 mm Falcon Primaria tissue culture dishes (Becton Dickinson Labware, Franklin Lakes, NJ, USA) containing 15 mL of diet. Diet dishes were replaced on alternate days without anesthesia (CO2) after tapping flies to the bottom of the bottle. Mortality was recorded daily and mortality calculations and Gompertz-Makeham maximum likelihood estimates done using WinModest (v.1.0.2) as described previously (Krishnan et al., 2009 (link)).
8 oz round bottom polypropylene bottles
Manufactured by Genesee Scientific
Sourced in United States
The 8 oz round bottom polypropylene bottles are laboratory equipment designed for various storage and containment purposes. These bottles are made of polypropylene, a durable and chemically resistant material, and have a round bottom shape. The bottles have a capacity of 8 fluid ounces and are suitable for a range of laboratory applications.
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Lab products found in correlation
3 protocols using 8 oz round bottom polypropylene bottles
1
Measuring Lifespan in Fruit Flies
2
Aging Drosophila Melanogaster Maintenance
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Flies were raised on standard cornmeal-yeast-molasses diet at 25∘C under a 12 h light/12 h dark (LD) regimen (where Zeitgeber time (ZT) 0 is time of lights on and ZT12 is time of lights off). Light intensity was kept at ∼1500 lux. Mated males were separated 1–2 days after emergence. Aging males were kept in inverted 8 oz round bottom polypropylene bottles (Genesee Scientific, San Diego, CA, USA) on 35 mm petri dishes (BD Falcon, San Jose, CA, USA) containing 15 ml of diet. Fresh diet was provided three times a week. All experiments were completed using the w1118 strain.
3
Lifespan Measurement of Drosophila
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