The measurement of CAT activity was conducted using Catalase Activity Colorimetric Assay Kit (Biovision®). The CAT activity was determined by measuring a reduced absorbance of H2O2 at 570 nm. Absorbance was measured at 570 nm using an ELISA.
Catalase activity colorimetric assay kit
Manufactured by Abcam
Sourced in United States
The Catalase Activity Colorimetric Assay Kit is a laboratory tool used to measure the activity of the enzyme catalase. Catalase is an important antioxidant enzyme found in most living organisms that catalyzes the decomposition of hydrogen peroxide (H2O2) into water and oxygen. The kit provides a colorimetric method to quantify catalase activity in various sample types.
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3 protocols using catalase activity colorimetric assay kit
1
Catalase Activity Colorimetric Assay
2
Antioxidant Enzyme Activity in Male Flies
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Newly eclosed male flies were pretreated with KRG 25 μg/mL for 7 days. Thirteen to thirty flies with three replicates were collected with CO2 anaesthetization. Activities of superoxide dismutase (SOD) and catalase (Cat) were measured using the SOD Assay Kit-WST (19160, Sigma-Aldrich) and Catalase Activity Colorimetric Assay Kit (BioVision, USA), respectively, as described by manufacturers. Statistical probability was measured using t-test.
3
Catalase Activity Colorimetric Assay
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The cells were lysed in cold assay buffer and centrifuged at 10,000 × g for 15 min at 4℃. The supernatants were harvested and placed in wells. The CAT activity was determined using the Catalase Activity Colorimetric assay kit (BioVision Inc. Mountain View, CA, USA) in accordance with the manufacturer's instruction [23 ]. The activity of CAT was measured at 570 nm using a microplate reader.
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