Ftir 8400s fourier transform infrared spectrometer

The Shimadzu FTIR-8400S Fourier Transform Infrared Spectrometer instrument used for analyzes the presence of functional groups in the isolated compound. The spectrometer works under purged condition. Solid sample of an isolated compound was dispersed in polyethylene pellets depending on the region of interest. This instrument has a typical resolution of 1.0/cm. Signal averaging, signal enhancement, baseline correction and other spectral manipulations were possible. Structure elucidations of the isolated compound were carried out using spectroscopic techniques: Mass spectrometry, ¹H and ¹³C NMR together with two-dimensional experiments (correlation spectroscopy [COSY] and heteronuclear single quantum coherence [HSQC]). Optical rotation was determined with a Perkin-Elmer polarimeter (model 341). NMR spectra on solutions in CDCl₃ were recorded on a Bruker DRX-500 NMR Spectrometer at 500 MHz (¹H) and 125 MHz (¹³C); the signals of the deuterated solvent were taken as a reference. The molecular mass of the isolated compound was analyzed by mass spectrometry.