Nicolet in10 mx ftir spectrometer

FTIR measurements were carried out using a NICOLET iN10 MX FTIR spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with a KBr/Ge beam splitter and a mercury cadmium telluride (MCT) detector. All spectra were recorded in the range of 4000–600 cm⁻¹ with a resolution of 4 cm⁻¹ and 16 scans. For each sample, we detected at least three times, and a representative spectrum was presented for the following analyses. Each spectrum was initially baseline corrected and normalized for the intensity spanning from 0 to 1 to obtain a better comparison between spectra. The experiment of capturing the spectra of cells with or without DHA treatment was also repeated at least three times.
To further compare the spectral differences between cells with and without DHA treatment, the spectrum of cells without DHA treatment was subtracted by the spectrum of cells of the same cell line treated with DHA for 48 hr. Thus, we obtained the subtraction spectra. Note that the subtraction spectra were obtained using preprocessed spectra, which were baseline corrected and normalized from 0 to 1.