Anti ki67 percp cy5.5 b56

Manufactured by BD

Anti-Ki67-PerCP-Cy5.5 (B56) is a fluorochrome-conjugated monoclonal antibody that targets the Ki-67 protein, a well-established marker of cellular proliferation. The PerCP-Cy5.5 fluorophore allows for the detection of Ki-67 expression using flow cytometry or other fluorescence-based techniques.

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2 protocols using anti ki67 percp cy5.5 b56

Multi-Marker Immunophenotyping of T Cells

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Anti-CD3-AlexaFluor®700 (UCHT1), anti-CD4-APC-eFluor®480 (RPAT4), anti-CD69-biotin (FN50), anti-CD127-PerCP-Cy5.5 (eBioRDRS), anti-CD27-APC-eFluor®780 (LG.7F9), anti-CD4-FITC (RPA-T4), anti-CD27-PE-Cy7 (LG.7F9), anti-CD45-APC (2D1), and anti-EpCAM-PE (IB7), anti-FoxP3-PE-Cy7 (PCH101), anti-IL-2-PE-Cy7 (MQ1-17H12), anti-IFNγ-APC-eFluor®780 (4S.B3), anti-TNFα-PerCP-Cy5.5 (Mab11), anti-PD-1-APC (MIH4), anti-PD-L1-PE-Cy7 (MIH1), mIgG1κ-PE-Cy7 (P3.6.2.8.1), anti-CD45RAFITC (HI100), anti-CD45RO-biotin (UCHL1), CD62L-PE-Cy7 (DREG-56), CCR7-APC-efl780 (3D12), anti-CD3 (OKT3), and anti-CD28 (CD28.2) were purchased from eBioscience (San Diego, CA). Anti-CD8-BV510 (RPA-T8) was purchased from BioLegend (San Diego, CA). Anti-CTLA-4-Biotin and anti-Ki67-PerCP-Cy5.5 (B56) were purchased from BD Biosciences (San Diego, CA). Anti-TIM-3-PE (344823) was purchased from R&D systems. Anti-LAG-3-FITC (17B4) was purchased from Enzo Life Sciences International, Inc. (Plymouth Meeting, PA). Anti-CD107a-PE-Cy7 (H4A3) and anti-perforin-PerCP-Cy5.5 (δG9) were purchased from BD Biosciences. Anti-galectin-9 (ab69630) was purchased from Abcam. Control rabbit IgG (BA-1000) was purchased from Vector Labs (Burlingame, CA). Goat-anti-Rabbit IgG-HRP was purchased from Dako (Carpinteria, CA). All antibodies were used per the manufacturer's recommendations.

Severson J.J., Serracino H.S., Mateescu V., Raeburn C.D., C.McIntyre R J.r., Sams S.B., Haugen B.R, & French J.D. (2015). PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer immunology research, 3(6), 620-630.

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Multiparametric Flow Cytometry of Activated Tregs

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Activated Tregs were stained with LIVE/DEAD® Fixable Dead Cell Stain kit (Invitrogen, Grand Island, NY), anti-CD4-AF700 (RPA-T4) and anti-CD25-allophycocyanin (BD-Biosciences) for surface marker. Then, cells were stained for intracellular markers using anti-CD152-PE (CTLA-4; 14D3), anti-Ki67-PerCP-Cy5.5 (B56) (BD-Biosciences) and anti-FOXP3-Pacific Blue (PCH101) (eBioscience). Flow cytometry analysis was performed using FACSDiva software version 6.1.2 (BD-Biosciences).

Rueda C.M., Moreno-Fernandez M., Jackson C.M., Kallapur S.G., Jobe A.H, & Chougnet C.A. (2015). Neonatal regulatory T cells have reduced capacity to suppress dendritic cell function. European journal of immunology, 45(9), 2582-2592.

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