Igor pro

Data are reported as Mean ± SEM or as percentage of totals for distributions. Comparisons were made using one-tailed ANOVA tests followed by Tukey’s HSD test for normal distributions in JMP software (SAS, Cary, NC, USA), or Student’s t-tests in Igor Pro (Wavemetrics, Portland, OR, USA). For non-normal distributions, we used Wilcoxon/Kruskal–Wallis multiple comparison tests followed by the Benjamini–Hochberg procedure using a false discovery rate (FDR) of 0.05 in JMP software (SAS, Cary, NC, USA) or a Mann–Whitney U test in Igor Pro. Multiple-comparison corrections were performed with Excel scripts. P-values of <0.05 were considered significant.

Loose-cell attached recordings were made with a glass electrode pulled with a P-1000 puller (Sutter Instruments, Novato, CA, USA) filled with ACSF to record action potentials without dialyzing the intracellular solution and thereby altering intrinsic firing rates. Data was collected and analyzed off-line using custom acquisition and analysis routines with Igor Pro software (Wavemetrics, Portland, OR, USA). Statistical comparisons were made using Igor Pro or JMP (SAS, Cary, NC, USA) software. Data are represented as mean ± standard error of the mean (SEM), N = animal number, n = cell number.