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Precast 4 20 tris hcl gels

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Precast 4–20% Tris-HCl gels are a type of laboratory equipment used for protein electrophoresis. They are pre-cast polyacrylamide gels with a gradient concentration of 4-20% acrylamide, designed for the separation and analysis of proteins based on their molecular weight.

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Lab products found in correlation

Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Image lab v4, by Bio-Rad (2 mentions) Precast tris tricine gels, by Bio-Rad (2 mentions)

Close spelling variants of this product

Tris-HCl precast gels 4–20% precast Tris-HCl gels 4–15% Tris-HCl gels Precast gels Tris gels Tris-HCl

2 protocols using precast 4 20 tris hcl gels

1

Quantitative Analysis of Exendin Derivatives

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Concentrations of fusion proteins were measured on a ND-1000 Nanodrop spectrophotometer (Thermo Scientific) by UV-vis absorption spectroscopy. Concentration of exendin and conjugates for in vitro assays and in vivo studies was assessed using a Bicinchoninic Acid (BCA, Pierce) assay following manufacturer’s instructions. SDS-PAGE analysis of sortase A was performed using precast 4–20% Tris-HCl gels (Bio-Rad). SDS-PAGE analyses of all exendin derivatives were performed using precast Tris/Tricine gels (Bio-Rad). Quantification of sortase reaction conversion was done by gel densitometry analysis using a built-in function in Image Lab (v. 4.0.1, Bio-Rad).
Qi Y., Simakova A., Ganson N.J., Li X., Luginbuhl K.M., Özer I., Liu W., Hershfield M.S., Matyjaszewski K, & Chilkoti A. (2016). A brush-polymer conjugate of exendin-4 reduces blood glucose for up to five days and eliminates poly(ethylene glycol) antigenicity. Nature biomedical engineering, 1, 0002.
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2

Quantitative Analysis of Exendin Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols
Concentrations of fusion proteins were measured on a ND-1000 Nanodrop spectrophotometer (Thermo Scientific) by UV-vis absorption spectroscopy. Concentration of exendin and conjugates for in vitro assays and in vivo studies was assessed using a Bicinchoninic Acid (BCA, Pierce) assay following manufacturer’s instructions. SDS-PAGE analysis of sortase A was performed using precast 4–20% Tris-HCl gels (Bio-Rad). SDS-PAGE analyses of all exendin derivatives were performed using precast Tris/Tricine gels (Bio-Rad). Quantification of sortase reaction conversion was done by gel densitometry analysis using a built-in function in Image Lab (v. 4.0.1, Bio-Rad).
Qi Y., Simakova A., Ganson N.J., Li X., Luginbuhl K.M., Özer I., Liu W., Hershfield M.S., Matyjaszewski K, & Chilkoti A. (2016). A brush-polymer conjugate of exendin-4 reduces blood glucose for up to five days and eliminates poly(ethylene glycol) antigenicity. Nature biomedical engineering, 1, 0002.
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