Harvested tissues were washed with CaSO4 (1 mM) and deionized water before drying in an oven at 80°C for 3 days (Kabir et al., 2015 (link)). Once tissues were dried, 3 mL HNO3 and 1 mL of H2O2 (hydrogen peroxide) were mixed with samples and heated at 75°C for 10 min. The concentration of Fe was then analyzed by Flame Atomic Absorption Spectroscopy (AAS) outfitted with an ASC-6100 autosampler and air-acetylene atomization gas mixture system (Model No. AA-6800, Shimadzu). Standard solutions of Fe were separately prepared from their respective concentration of stock solutions (Shimadzu).
Asc 6100
Manufactured by Shimadzu
Sourced in Japan
The ASC-6100 is an analytical scale from Shimadzu designed for laboratory use. It provides accurate and precise weight measurements. The core function of the ASC-6100 is to weigh samples with a high degree of accuracy.
Automatically generated - may contain errors
8 protocols using asc 6100
1
Iron Quantification in Plant Tissues
2
Comprehensive Milk Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pH was measured on a pH meter and acidity was determined by the titration method using 0.11 N NaOH solution. Fat, protein, lactose and total solids contents were determined by means of infrared spectroscopy (Milkoscan FT6000, Foss, Hillerod, Denmark). Ash content was determined by the AOAC method [18 ]. Total nitrogen (TN) as well as water soluble nitrogen (WSN) were determined by the Kjeldahl method. Phosphorus content was determined by molecular absorption spectrometry [19 ]. Moreover, calcium content of ovine milk was measured by Atomic Absorption Spectrometry [20 ] on a Shimadzu AA-6800 Atomic Absorption Spectrophotometer (Shimadzu, Kyoto, Japan) equipped with the autosampler Shimadzu ASC-6100 and the software WizAArd v. 2.30.
Somatic cell counts (SCC) were determined on Fossomatic (Foss, Hillerod, Denmark). All analyses were performed in duplicate.
Somatic cell counts (SCC) were determined on Fossomatic (Foss, Hillerod, Denmark). All analyses were performed in duplicate.
3
Analytical Methods for Brine-Salted Cheeses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before analyses, the brine-salted cheeses were removed from the brine and drained for 2 min. All cheeses were analyzed for the total solids content by heating at 102 °C [12 ], and for the ash content as specified in the AOAC method [13 ]. Acidity was determined by a titration method using 0.11 N NaOH solution. The NaCl content was determined by the potentiometric titration method [14 ]. Calcium, magnesium, potassium, and sodium contents were determined by the Atomic Absorption Spectrometry method [15 ] on a Shimadzu AA-6800 Atomic Absorption Spectrophotometer (Shimadzu AA-6800, Kyoto, Japan) equipped with the autosampler Shimadzu ASC-6100 and the software WizAArd v. 2.30. Phosphorus content was determined by the molecular absorption spectrometry [16 ]. All analyses were performed in duplicate.
4
Determination of Cd and Fe in Plants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Harvested roots and shoots were washed with CaSO4 and deionized water before drying in an oven at 80°C for 3 days. The dried samples were then digested in 3 mL HNO3 before heating at 75°C for 10 min. Further, 1 mL of H2O2 was added to each vessel through the ventilation hole and then heated at 109°C for 15 min. The samples were then analyzed for Cd and Fe concentrations by Flame atomic absorption spectroscopy (AAS) outfitted with the ASC-6100 autosampler and air-acetylene atomization gas mixture system (Model No. AA-6800, Shimadzu). Standard solutions of Cd and Fe were prepared separately from their respective concentrations of stock solutions to cover the optimum absorbance ranges for the standard calibration curve.
5
Trace Element Analysis by AAS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An atomic absorption spectrophotometer (AAS-6800 Shimadzu, Koyoto, Japan) coupled with flame atomic absorption spectrometry (FAAS), GFA-EX7 graphite furnace atomizer (GFA), and ASC-6100 auto sampler (Shimadzu, Koyoto, Japan) utilized for trace element analyses. A high-density graphite tube used for atomization of trace elements and. hollow cathode lamps used for irradiation. FAAS with employed with air/acetylene (10/1.5) and nitrous oxide acetylene flams for the determination of Mo with the burner position adjusted for maximum sensitivity.
6
Determination of Zn and Fe Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In accordance with Kabir et al.60 (link), the concentration of Zn an Fe were determined. Initially, plant tissues (both root and shoot) were collected and washed with 1 mM CaSO4 for 5 min followed by a thorough rinse with distilled water. The cleaned samples were then dried in an incubator at 80 °C for 4 days. After the drying process was completed, the plant tissues were mixed with 3 mL HNO3 and 1 mL H2O2 in a test tube and heated at 75 °C for 12 min. The concentrations of Zn and Fe were examined through Flame Atomic Absorption Spectroscopy, employing an ASC-6100 auto sampler and an air-acetylene atomization gas mixture system (Model No. AA-6800, Shimadzu). Standard solutions of Zn and Fe were individually prepared to establish their respective concentrations.
7
Measuring Zn and Fe in Plant Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of Zn and Fe were determined according to Kabir et al. 201767 (link). For this, fresh plant tissues were washed with CaSO4 (1 mM) for 5 min and then thoroughly washed with distilled water. After this, clean samples were dried in an incubator at 80 °C for 6 days. A total of 3 mL of HNO3 and 1 mL of H2O2 were added to each sample in the test tube and heated at 75 °C for 12 min. Zn and Fe concentrations were evaluated by Flame Atomic Absorption Spectroscopy, using a ASC-6100 auto sampler and air-acetylene atomization gas mixture system (Model No.AA-6800, Shimadzu), where standard solutions of Zn and Fe were separately prepared from their respective concentrations.
8
Atomic Absorption Spectrophotometry Procedure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the experimental work was carried out on a Shimadzu Atomic Absorption Spectrophotometer (AAS)-6300 230V system, equipped with electrothermal graphite furnace atomization (Shimadzu, EX7i, Tokyo, Japan), an auto-sampler (Model ASC-6100), a deuterium arc background correction system, and a data processing unit. A Hamamatsu Deuteurium lamp, a Hamamatsu Al Hollow-Cathode Lamp, a pyrolytically coated graphite tube (64F7280801), a re-circulating water cooler, and a fume extraction system were used. Creatinine measurement was done using a Shimadzu UV-Visible spectrophotometer (Model 2450).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!