Anti cytl1 antibody

Recombinant human Cytl1 (donated by the School of Basic Medical Science, Peking University) was dissolved in 100% ethanol to prepare a stock solution of 360 ng/μl and further diluted to final concentrations ranging from 0.1 ng/ml to 10 ng/ml[6 (link)] using appropriate cell culture media. Progesterone and estradiol (Sigma, Louis, MO, USA) was dissolved to prepare a stock solution of 0.5 mM and used at final concentrations ranging 10 μM to 50 μM. Anti-Cytl1 antibody (Abcam-129767 Cambridge, UK) was used at a final concentration 1 μg/ml.

Oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone, antimycin, and CHX were purchased from Sigma-Aldrich (St. Louis, MO). Decitabine, monensin, MG132, KX2-391, and IACS-010759 were from Selleck (Houston, TX). Recombinant human CYTL1 was purchased from OriGene Technologies (Rockville, MD). Anti-CYTL1 antibody was purchased from Abcam (Cambridge, UK). Anti-NDUFV1, anti-BRCA1, anti-Flag, anti-GAPDH, and anti-Src antibodies were from Proteintech Group (Chicago, IL). Anti-HA, anti-CD31, anti-MDM2, anti-LDHA, anti-p-LDHA (Y10), and anti-COX4 antibodies were from Cell Signaling Technology (Beverly, MA). Anti-Tubulin, anti-β-Actin, anti-myc, and p-Src (Santa Cruz) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Lipofectamine 3000 and Lipofectamine RNAi MAX were purchased from Thermo Fisher Scientific (Waltham, MA). MitoCheck Complex I activity assay kit (No.700930) was purchased from Cayman Chemical (Ann Arbor, Michigan). Human CYTL1 ELISA Kit was purchased from CUSABIO Life Sciences (College Park, MD).