Oxldl elisa kit

Manufactured by Cusabio

Sourced in United States

The OxLDL ELISA kit is a laboratory equipment used for the quantitative determination of oxidized low-density lipoprotein (OxLDL) in samples. It is an enzyme-linked immunosorbent assay (ELISA) technique that utilizes specific antibodies to detect and measure OxLDL levels.

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Lab products found in correlation

Multiplex immunoassay kit, by Mesoscale (1 mentions) Il 10 elisa kit, by Thermo Fisher Scientific (1 mentions) Chemwell 2910 analyzer, by Awareness Technology (1 mentions) Human il 8 elisa kit, by Thermo Fisher Scientific (1 mentions)

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ELISAs

3 protocols using oxldl elisa kit

Multiplex Assays for Plasma Cytokines

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Cytokine levels in plasma and media were measured using the following immunoassay kits: multiplex immunoassay kit (Meso Scale Discovery); IL-10 ELISA kit (Thermo Scientific); IL-4, IL-17, TGF-β and IFN-γ ELISA kit (eBioscience). Plasma lipids were measured using an enzymatic assay (Wako Chemicals USA, Inc.) on a ChemWell 2910 analyzer (Awareness Technology, Inc). Oxidized LDL cholesterol was measured using the OxLDL ELISA kit (CUSABIO Life Science).

Kang J.G., Sung H.J., Amar M.J., Pryor M., Remaley A.T., Allen M.D., Noguchi A.C., Springer D.A., Kwon J., Chen J., Park J.H., Wang P.Y, & Hwang P.M. (2016). Low ambient oxygen prevents atherosclerosis. Journal of molecular medicine (Berlin, Germany), 94(3), 277-286.

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Quantification of Plasma Inflammatory Markers

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OxLDL was measured in plasma one week before sacrifice using an oxLDL ELISA kit according to manufacturer’s instructions (CSB-E07933m, CUSABIO, Houston, TX, USA). In the same plasma samples, MIF was quantified with the MIF DuoSet ELISA kit (DY1978, R&D Systems, Minneapolis, MN, USA). The concentrations of MCP-1 and adhesion molecules were analyzed in plasma as described previously [64 (link)]. Briefly, ELISAs were performed to measure MCP-1, E-selectin, P-selectin, ICAM-1 and VCAM-1 (with MCP-1 DuoSet (DY479-05), E-selectin/CD26E DuoSet (DY575), P-selectin/CD62P DuoSet (DY737), ICAM-1/CD54 DuoSet (DY796), VCAM-1/CD106 DuoSet, (DY643), respectively, all from R&D Systems).

Seidel F., Kleemann R., van Duyvenvoorde W., van Trigt N., Keijzer N., van der Kooij S., van Kooten C., Verschuren L., Menke A., Kiliaan A.J., Winter J., Hughes T.R., Morgan B.P., Baas F., Fluiter K, & Morrison M.C. (2022). Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice. International Journal of Molecular Sciences, 23(18), 10736.

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Quantifying IL-8 and ox-LDL in Cells

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Human IL-8 ELISA kit (Invitrogen, U.S.A.) and ox-LDL ELISA kit (Cusabio Technology LLC, U.S.A.) were use to determine concentrations of IL-8 and ox-LDL respectively in cell lysate and conditional media samples in accordance to the manufacturer’s instructions.

Pek N.M., Phua Q.H., Ho B.X., Pang J.K., Hor J.H., An O., Yang H.H., Yu Y., Fan Y., Ng S.Y, & Soh B.S. (2019). Mitochondrial 3243A > G mutation confers pro-atherogenic and pro-inflammatory properties in MELAS iPS derived endothelial cells. Cell Death & Disease, 10(11), 802.

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