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Nexera x2 ultra hplc

Manufactured by Shimadzu
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Sourced in Japan

The Nexera X2 ultra HPLC is a high-performance liquid chromatography system designed for analytical and preparative applications. It features a compact design, high-speed data processing, and advanced control software to provide efficient and reliable separation and detection of a wide range of chemical compounds.

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Lab products found in correlation

Lc solutions software, by Shimadzu (2 mentions) Lcms 8050 triple quadrupole mass spectrometer, by Shimadzu (2 mentions) Luna c18, by Phenomenex (1 mentions) Accq tag derivation kit, by Waters Corporation (1 mentions) Zorbax eclipse plus c18, by Agilent Technologies (1 mentions)

3 protocols using nexera x2 ultra hplc

1

Quantification of Plasma Melatonin Levels

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Measurement of plasma MEL levels was carried out as per the method reported previously with some modifications.24 Briefly, MEL levels in plasma samples were measured using a validated LC/MS-MS using a Shimadzu-Nexera X2 ultra HPLC consisting of binary gradient pumps (LC-30AD), auto sampler (SIL-30AC), mobile phase degasser (DGU20ASR), and a column oven (CTO-20AC) coupled with an LCMS-8050 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan). The data were analyzed using LC Solutions software (Shimadzu, Kyoto, Japan). The parameters were adjusted to yield maximum multiple reaction monitoring signals. The Q1/Q3 for MEL was set at 304.80 > 288.10 m/z and 182.70 > 119.80 m/z for IS NPEA in the positive electrospray ionization mode, respectively. Chromatographic separation of the analytes was done using Luna C18 (4.6 × 150 mm, 5 μm, Phenomenex, USA) protected with a C18 guard column from the same source. The liquid chromatography conditions were as follows: solvent A: water containing 0.1% formic acid and solvent B: 0.1%; formic acid in acetonitrile was used as mobile phase with gradient elution of solvent B at 20% (0-4.0 minutes); 80% (4.0-6.0 minutes); 20% (7.0-10.0 minutes) at a flow rate of 0.8 mL/min. The total run time was 10 minutes. Retention time for MEL was 2.2 minutes and the IS was 2.7 minutes. The concentration of MEL was expressed as ng/mL.
Pai A.A., Devasia A.J., Panetta J.C., Mani S., Illangeswaran R.S., Mohanan E., Balakrishnan B., Lakshmi K.M., Kulkarni U., Aboobacker F.N., Korula A., Abraham A., Srivastava A., Mathews V., George B, & Balasubramanian P. (2019). Pharmacokinetics and Efficacy of Generic Melphalan Is Comparable to Innovator Formulation in Patients With Multiple Myeloma Undergoing Autologous Stem Cell Transplantation. Clinical lymphoma, myeloma & leukemia, 20(2), 130-135.e1.
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2

Quantification of Target Amino Acids

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Target amino acids were quantified using Waters AccQ-Tag derivation kit with 20 μl serum by A Nexera X2 ultra HPLC equipped with a triple quadrupole 8050 (Shimadzu). The details of analytical condition as described in previous study61 (link).
Li H., Zhang L., Li J., Wu Q., Qian L., He J., Ni Y., Kovatcheva-Datchary P., Yuan R., Liu S., Shen L., Zhang M., Sheng B., Li P., Kang K., Wu L., Fang Q., Long X., Wang X., Li Y., Ye Y., Ye J., Bao Y., Zhao Y., Xu G., Liu X., Panagiotou G., Xu A, & Jia W. (2024). Resistant starch intake facilitates weight loss in humans by reshaping the gut microbiota. Nature Metabolism, 6(3), 578-597.
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3

Quantification of Treosulfan and EBDM

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Treosulfan and S, S-EBDM levels in plasma were measured using a Shimadzu-Nexera X2 ultra HPLC coupled with an LCMS-8050 triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan). The parameters were adjusted to yield maximum multiple reaction monitoring signals (Table S1). Chromatographic separation of the analytes was done using Zorbax Eclipse Plus C18 (100 mm × 2.1 mm, 3.5 μm; Agilent, CA) protected with a C18 guard column from the same source using isocratic elution with a mobile phase 0.01 M ammonium formate buffer at a flow rate of 0.4 mL/minute maintained at 40°C. The total run time was 5.5 minutes (Figure S1). The chromatograms were analyzed using LC Solutions software (Shimadzu, Kyoto, Japan).
Pai A.A., Mohanan E., Panetta J.C., Kulkarni U.P., Illangeswaran R.S., Balakrishnan B., Jayaraman A., Edison E.S., Lakshmi K.M., Devasia A.J., Fouzia N.A., Korula A., Abraham A., George B., Srivastava A., Mathews V., Standing J.F, & Balasubramanian P. (2023). Treosulfan Exposure Predicts Thalassemia-Free Survival in Patients with Beta Thalassemia Major Undergoing Allogeneic Hematopoietic Cell Transplantation. Clinical pharmacology and therapeutics, 115(1), 116-125.
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