Cells were seeded into six-well plates at a density of 1 × 106 cells in 2 ml RPMI 1640 medium per well and cultured overnight. Cells were then treated with or without 10 μM KU60019 and maintained at 1% O2 for 10–12 h. Glucose consumption, lactate production, ATP production, citrate production, succinate production, fumarate production, and PFKP and CS activity were detected using glucose assay kit (Solarbio® BC2500), LA assay kit (Solarbio® BC2230), ATP content assay kit (Solarbio® BC0300), citric acid (CA) content assay (Solarbio® BC2150), micro mitochondrial citric acid content assay kit (Solarbio® BC2175), succinate colorimetric assay kit (Sigma® MAK184), pyruvate (PA) assay kit (Solarbio® BC2200), acetyl-CoA assay kit (Solarbio® BC0980), fumarate assay kit (Sigma® MAK060), PFKP test kit (Nanjing Jiancheng Bioengineering Institute A129), and citroyl synthetase kit (Nanjing Jiancheng Bioengineering Institute A108) according to instruction of the manufacturers, respectively. All experiments were performed at least three times and the data were normalized by the cell numbers or protein content.
Pfkp test kit
Manufactured by Nanjing Jiancheng
The PFKP test kit is a laboratory equipment product designed to detect the presence and measure the levels of the enzyme phosphofructokinase (PFKP) in biological samples. The core function of the PFKP test kit is to provide researchers and scientists with a reliable method for analyzing PFKP activity, which is an important step in various biochemical and metabolic studies.
Automatically generated - may contain errors
Lab products found in correlation
Citric acid content assay, by Solarbio (2 mentions)
Glucose assay kit, by Solarbio (2 mentions)
Fumarate assay kit, by Merck Group (2 mentions)
Micro mitochondrial citric acid content assay kit, by Solarbio (2 mentions)
Citroyl synthetase kit, by Nanjing Jiancheng (2 mentions)
Acetyl coa assay kit, by Solarbio (2 mentions)
Succinate colorimetric assay kit, by Merck Group (2 mentions)
Atp content assay kit, by Solarbio (2 mentions)
La assay kit, by Solarbio (2 mentions)
2 protocols using pfkp test kit
1
Metabolic Profiling of Cells under Hypoxia
2
Metabolic Profiling of Cells under Hypoxia
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Cells were seeded into six-well plates at a density of 1 × 106 cells in 2 ml RPMI 1640 medium per well and cultured overnight. Cells were then treated with or without 10 μM KU60019 and maintained at 1% O2 for 10–12 h. Glucose consumption, lactate production, ATP production, citrate production, succinate production, fumarate production, and PFKP and CS activity were detected using glucose assay kit (Solarbio® BC2500), LA assay kit (Solarbio® BC2230), ATP content assay kit (Solarbio® BC0300), citric acid (CA) content assay (Solarbio® BC2150), micro mitochondrial citric acid content assay kit (Solarbio® BC2175), succinate colorimetric assay kit (Sigma® MAK184), pyruvate (PA) assay kit (Solarbio® BC2200), acetyl-CoA assay kit (Solarbio® BC0980), fumarate assay kit (Sigma® MAK060), PFKP test kit (Nanjing Jiancheng Bioengineering Institute A129), and citroyl synthetase kit (Nanjing Jiancheng Bioengineering Institute A108) according to instruction of the manufacturers, respectively. All experiments were performed at least three times and the data were normalized by the cell numbers or protein content.
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