G series media

Manufactured by Vitrolife

Sourced in Sweden

Vitrolife G-series media is a range of culture media designed for use in assisted reproductive techniques. The media are formulated to provide a stable and balanced environment for the culture of gametes and embryos.

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Lab products found in correlation

G 1 plus, by Vitrolife (1 mentions) G2 plus, by Vitrolife (1 mentions)

2 protocols using g series media

In Vitro Fertilization Procedure

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The protocol of semen/cumulus-oocyte complexes (COCs) preparation, insemination, and embryo culturing was performed as described in our previous paper (20 (link)). Density gradient centrifugation was used to optimize the semen samples. The concentration, motility, and morphology of sperm were evaluated according to the fifth edition of World Health Organization (WHO) guidelines. In IVF cycles, each COC was inseminated with 10,000 motile spermatozoa 3–4 h after retrieval. In intracytoplasmic sperm injection (ICSI) cycles, the COCs were denuded 2 h later, and sperm was injected 4 h after retrieval. Resulting zygotes were transferred to G1 Plus (Vitrolife, Sweden) and cultured in a time-lapse incubation system. The system was set to take pictures of each embryo every 10 min. Pronuclei were checked 16–18 h after insemination. On day 3, the media was replaced by G2 Plus (Vitrolife, Sweden). On days 5 and 6, blastocysts were cryopreserved for further use. All culture media used were Vitrolife G-series media (Vitrolife, Sweden).

Ma B.X., Yang L., Tian Y., Jin L, & Huang B. (2022). Cytoplasmic strings between ICM and mTE are a positive predictor of clinical pregnancy and live birth outcomes: A time-lapse study. Frontiers in Medicine, 9, 934327.

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ICSI with Patient Sperm

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ICSI was performed as described previously.11 (link) The metaphase II oocyte was injected with the sperm head and detached tail from the patient. The fertilization rate was assessed, and the embryos were cultured with Vitrolife G-Series media (Vitrolife, Goteborg, Sweden). The embryo quality was evaluated as described previously.12 (link)

Li Y.Z., Li N., Liu W.S., Sha Y.W., Wu R.F., Tang Y.L., Zhu X.S., Wei X.L., Zhang X.Y., Wang Y.F., Lu Z.X, & Zhang F.X. (2021). Biallelic mutations in spermatogenesis and centriole-associated 1 like (SPATC1L) cause acephalic spermatozoa syndrome and male infertility. Asian Journal of Andrology, 24(1), 67-72.

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