Tissue specimens were fixed in 10% formaldehyde, embedded in paraffin, and cut into 3‐μm thick sections. Each section was further stained with hematoxylin and eosin. Immunohistochemical staining was performed on paraffin sections using antibodies against CD3 (1:200, LN10; Novocastra), CD5 (1:100, 4C7; Novocastra), CD10 (1:100, 56C6; Novocastra), CD15 (1:50, Carb‐3; DAKO), CD20 (1:100, L26; DAKO), CD30 (1:40, Ber‐H2; DAKO), CD79a (1:100, JCB117; DAKO), Ki‐67 (1:2500, MIB‐1; DAKO), and PD‐L1 (1:400, E1LN3; Cell Signaling Technology) and samples were analyzed using a Bond III Stainer (Leica Biosystems).
Carb 3
Manufactured by Agilent Technologies
The Carb-3 is a laboratory instrument designed for the analysis of carbonate content in various materials. It provides accurate and reliable measurement of carbonate concentrations. The core function of the Carb-3 is to determine the carbonate composition of samples through standardized analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Pg m1, by Agilent Technologies (2 mentions)
Mib 1, by Agilent Technologies (1 mentions)
Bond 3 stainer, by Leica (1 mentions)
Ber h2, by Agilent Technologies (1 mentions)
Jcb117, by Agilent Technologies (1 mentions)
Ab215206, by Abcam (1 mentions)
Ab183322, by Abcam (1 mentions)
Ab52478, by Abcam (1 mentions)
Vectamount, by Vector Laboratories (1 mentions)
P syk, by Cell Signaling Technology (1 mentions)
T syk, by Santa Cruz Biotechnology (1 mentions)
3 protocols using carb 3
1
Immunohistochemical Analysis of Tissue Samples
2
Immune Cell Profiling Using Antibody Panel
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The following antibodies were used in this study: anti-CD11b (ab52478, Abcam), anti-CD4 (4B12, DAKO), anti-CD8 (C8/144B, DAKO), anti-CD14 (ab183322, Abcam), anti-CD15 (Carb-3, DAKO), anti-CD33 (17425-1-AP, Proteintech), anti-HLA-DR (17221-1-AP, Proteintech), anti-CD20 (L26, DAKO), anti-FOXP3 (ab215206, Abcam), and anti-CD68 (PG-M1, DAKO).
3
Immunohistochemical Analysis of Syk Protein
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Immunohistochemistry (IHC) was performed on formalin‐fixed paraffin‐embedded (FFPE) tissues. Primary antibodies were T‐Syk (diluted 1:500, N‐19; Santa Cruz Biotechnology) or P‐Syk (diluted 1:25, Tyr525/526; Cell Signaling Technology), followed by a secondary polymeric horseradish peroxidase system (EnVision; Dako) and 3,3‐diaminobenzidine to develop. For double‐staining, primary antibodies used were MPO (diluted 1:1,000; Dako), CD15 (diluted 1:50, Carb‐3; Dako), or CD68 (diluted 1:50, PGM1; Dako). StayGreen AP Plus detection kit (Abcam) was used to develop sections, and slides were counterstained with Mayer's hematoxylin and mounted using Vectamount (Vector Labs).
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