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Penicillin streptomycin amphotericin b antibiotics

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Sourced in Israel

Penicillin–streptomycin–amphotericin B antibiotics are a combination of three different antibiotics used in cell culture applications. The core function of this product is to provide broad-spectrum antimicrobial protection against bacterial and fungal contamination in cell culture media and samples.

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Lab products found in correlation

Dmem f12, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)

Close spelling variants of this product

Penicillin (457 citations) Streptomycin (447 citations) Penicillin/streptomycin (382 citations) Amphotericin B (52 citations) Penicillin–streptomycin–amphotericin B (20 citations) Amphotericin (19 citations) Penicillin-streptomycin antibiotics (8 citations) Penicillin–streptomycin–amphotericin (5 citations)

2 protocols using penicillin streptomycin amphotericin b antibiotics

1

Cell Culture Protocols for HeLa and MDA-MB-231

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Wild-type cell lines were obtained from American Type Culture Collection (ATCC), Manassas, VA, USA. HeLa and MDA-MB-231 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, St Louis, MO, USA) and DMEM-F12, respectively. Both media were supplemented with 10% foetal bovine serum and penicillin–streptomycin–amphotericin B antibiotics (Biological Industries, Beit HaEmek, Israel). Cells were cultured in a 5% CO2 atmosphere at 37 °C.
Bravo-Sagua R., Parra V., Ortiz-Sandoval C., Navarro-Marquez M., Rodríguez A.E., Diaz-Valdivia N., Sanhueza C., Lopez-Crisosto C., Tahbaz N., Rothermel B.A., Hill J.A., Cifuentes M., Simmen T., Quest A.F, & Lavandero S. (2018). Caveolin-1 impairs PKA-DRP1-mediated remodelling of ER–mitochondria communication during the early phase of ER stress. Cell Death and Differentiation, 26(7), 1195-1212.
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2

HeLa Cell Line Maintenance Protocol

Cited 1 time
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Wild-type HeLa (CCL-2) cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA). Cells were maintained in DMEM supplemented with 10% FBS, 1 mM pyruvate, and Penicillin-Streptomycin-Amphotericin B antibiotics (Biological Industries, Beit-Haemek, Israel), as described before [7 (link), 8 (link)]. Cells were cultured in a 5% CO2 atmosphere at 37 °C and were used in passages 3–12.
Lopez-Crisosto C., Díaz-Vegas A., Castro P.F., Rothermel B.A., Bravo-Sagua R, & Lavandero S. (2021). Endoplasmic reticulum−mitochondria coupling increases during doxycycline-induced mitochondrial stress in HeLa cells. Cell Death & Disease, 12(7), 657.
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