For the Cellomics assay, cells were cultured in a 96-well plate for 24 h after lentiviral infection prior to experimentation. Green fluorescence protein (GFP)-expressing cells were quantified once daily for five days using a Cellomics ArrayScan system (Thermo Scientific, Waltham, MA, USA). For the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, cells were cultured in a 96-well plate for 24 h after lentiviral infection prior to experimentation. At the indicated time points, medium was replaced with fresh medium supplemented with MTT (0.5 mg/ml) (Sigma). Absorbance was measured at a wavelength of 490 nm (OD490).
Cellomics arrayscan system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Cellomics ArrayScan system is a high-content screening platform designed for automated microscopy and image analysis. It provides quantitative, high-throughput data on cellular and subcellular parameters.
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Lab products found in correlation
2 protocols using cellomics arrayscan system
1
Lentiviral Infection Assays for Cell Viability
2
VOPP1 Knockdown Impacts SMMC-7721 Cell Proliferation
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SMMC-7721 cells transfected with NC or VOPP1 KD lentivirus were then seeded in 96-well plates. Lentivirus-derived green fluorescent protein expression was observed by fluorescence microscopy. SMMC-7721 cells were grown to 70% to 90% confluency, then collected for further experiments. We investigated 2000 cells/well per day using the Cellomics ArrayScan System (Thermo Fisher Scientific). Subsequently, we measured them to determine the green fluorescence signal by changing the input parameters. Results were obtained for data analysis through a 5-day cell proliferation curve. According to this, genes were inferred to be proliferation-related if their silencing resulted in a fold-change >2 compared with the control group. The number of cells on the scanned image were counted using a Celigo Image Cytometer (Nexcelom, Lawrence, MA, USA).
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