Mouse anti flag sc 7392

Human embryonic kidney 293T cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS) and tested for mycoplasma contamination periodically by a PCR-based mycoplasma detection kit (Beyotime Biotec).
For immunoblot analysis, the following antibodies were used: Mouse anti-Flag (sc-7392, Santa Cruz Biotechnology), Rabbit anti-HA (H6908, Sigma-Aldrich). Rabbit anti-Myc (16286-1), anti-V5 (14440-1), Mouse anti-GFP (66002-1), Mouse anti-GAPDH (60004-1), Mouse anti-Tubulin (66031-1), goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP were from Proteintech. Rabbit anti-Flag (14793S), anti-HA (3724S), anti-p65 (8284S), anti-IRF3 (11904T) were purchased from Cell Signaling Technology. Rabbit anti-STAT1 (AF0288) and anti-LaminB1 (AF5222) were from Beyotime Biotec. Mouse anti-HA (201113) and anti-β-Actin (200068-8F10) antibodies were purchased from Zen-Bioscience.

The following antibodies were used in this study: rabbit anti-KAP1 (AF2620, Beyotime Biotec), rabbit anti-HA (H6908, Sigma-Aldrich). Rabbit anti-MAVS (14341-1), rabbit anti-RIG-I (20566-1), rabbit anti-Myc (16286-1), rabbit anti-Flag (20543-1), mouse anti-TRAF6 (66498-1), mouse anti-GAPDH (60004-1), mouse anti-Tubulin (66031-1), goat anti-mouse IgG-HRP, and goat anti-rabbit IgG-HRP were from Proteintech. Mouse anti-MAVS (sc-166583) and mouse anti-Flag (sc-7392) were from Santa Cruz Biotechnology. Mouse anti-HA (201113), mouse anti-β-Actin (200068-8F10), and rabbit anti-IRF3 (381333) antibodies were purchased from Zen-Bioscience. Rabbit anti-HA (3724S), anti-TBK1 (3504S), anti-p-TBK1 (5483SS), anti-p-IRF3 (29047S), anti-p-IκBα (2859S), and mouse anti-IκBα (4814S) were purchased from Cell Signaling Technology.
SeV was obtained as previously described and the multiplicity of infection was 1. Proteasome inhibitor MG132 (S1748, Beyotime Biotec) and (S7418, Selleck) were dissolved in dimethyl sulfoxide (D8418, Sigma-Aldrich). The autolysosome inhibitor bafilomycin A1 (BafA1; 88899-55-2, Aladdin) was kindly provided by Prof. Rui Cheng (Chongqing Medical University). MG132 (10 μM) and BafA1 (5 nM) were used for 6 h, and CHX was used at 100 μg/ml for indicated time.