Strains carrying the indicated luminescence reporters were grown from a single colony isolated over LB plates to a mid-logarithmic phase of growth. Cells were then grown in 300 μl MSgg medium, in a 96 well plate with white opaque walls and clear tissue culture treated flat bottoms (Corning). Measurements were performed every 30 min at 30 °C for 30 h, using a microplate reader (Synergy 2; BioTek, Winooski, VT, USA). Luciferase activity was calculated as RLU/OD, to avoid artefacts related to the luminescence intensity influenced by the bacterial growth rate.
Flat bottoms
Manufactured by Corning
Flat bottoms are a type of laboratory equipment designed to provide a stable and level surface for various scientific experiments and procedures. They are commonly used to hold samples, solutions, or other materials during laboratory work. Flat bottoms offer a reliable and consistent platform to ensure accurate and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using flat bottoms
1
Bacterial Luminescence Monitoring Protocol
2
Luminescence-based Growth Dynamics
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Strains carrying the indicated luminescence reporters were grown from a single colony isolated over LB plates to a mid-logarithmic phase of growth. Cells were then grown in 300 μl MSgg medium, in a 96 well plate with white opaque walls and clear tissue culture treated flat bottoms (Corning). Measurements were performed every 30 min at 30°C for 30 h, using a microplate reader (Synergy 2; BioTek, Winooski, VT, USA). Luciferase activity was calculated as RLU/OD, to avoid artefacts related to the normalization of luminescence intensity to the population size.
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