This cannulation approach was performed in the same manner as the above-written method for type A cannulation, but following that procedure an 18G needle was used to puncture the LV and a 20G, 5–8 mm long bulb tip catheter (FTP-20–30, Instech laboratories) was inserted into the LV. Afterward, 5 mL of UW and 125 IU of heparin were injected through the cannula inside the ascending aorta and the heart was placed in a container of cold UW solution.
Other tested cannulation methods include the following: Type A cannulation with 20G bulb tip catheter insertion into the LV via the left atrial appendage puncture, and after successful completion of type A cannulation, a small defect was created on the left atrial appendage and the mitral valve of the heart was removed using a 3 mm aortic punch (Medtronic, USA).
Other tested cannulation methods include the following: Type A cannulation with 20G bulb tip catheter insertion into the LV via the left atrial appendage puncture, and after successful completion of type A cannulation, a small defect was created on the left atrial appendage and the mitral valve of the heart was removed using a 3 mm aortic punch (Medtronic, USA).