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2 protocols using anti oas1

1

Antibody Acquisition for Protein Analysis

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Anti-PKR, anti-eiF2, anti-IRF3, anti-IRF9, anti-NF-kB and anti-OAS1 antibodies were obtained from Santa Cruz; the anti-phospho-PKR antibody was obtained from Abcam; anti-phospho-eiF2, anti-STAT1, anti-phospho-STAT1 antibody were obtained from Cell Signaling Technologies; the anti-NS5A antibody (9E10) was generously obtained by Dr. C. Rice; and anti-calnexin antibody was obtained from Sigma.
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2

OAS1 Knockdown and Protein Analysis

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The cells were transfected with OAS1 specific siRNA for 72 h and then lysed with RIPA (medchemexpress), then protein lysates were boiled at 100˚C with 5×loading buffer for 6 min. The cell lysates were separated using 10% SDS-PAGE and transfer to the PVDF membranes (Millipore, USA) (24 (link)). The PVDF membranes were blocked with 5% defatted milk for 2 h. The anti-OAS1 (Santa Cruz, CA, sc-374656, 1:2000 dilution), anti-GAPDH (BOSTER, China, A00227-1, 1:4000 dilution) were used as primary antibody. HRP-conjugated goat anti-mouse (BOSTER, China, BA1051, 1:5000 dilution) was used as secondary antibody. ImageJ software was used for stripe analysis.
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