1525 2707 2489 series hplc system

Analysis of constituents in SLBZS was performed on an 1525-2707-2489 series HPLC system (Waters, United States) equipped with a binary solvent manager, sample manager, column compartment, UV detector with 280 nm, and LC-Solution software. The chromatographic column is Sun Fire C18 column (4.6 × 250 mm, 5 μm). Mobile phase A was 0.1% phosphoric acid aqueous solution (1:999, V/V), and mobile phase B was acetonitrile, the gradient elution procedure is shown in Supplementary Table S1. The flow rate was 1 mL /min, the injection volume was 50 μL, the column temperature is 40°C, Uv detection wavelength was selected as 203 nm. HPLC diagram of ginsenoside Rb1 standard was shown in Supplementary Figure S1.

Analysis of constituents in FF was performed on an 1525-2707-2489 series HPLC system (Waters, United States) equipped with a binary solvent manager, sample manager, column compartment, UV detector with 280 nm, and LC-Solution software. The separation was performed on an Agilent TC-C₁₈ column (5 μm, 4.6 × 250 mm). The mobile phase consisted of water containing 0.1% acetic acid (A) and acetonitrile (B). The linear gradient was as follows: 0–30 min, 10–25% B; 30–45 min, 25–75% B; and 45–50 min, 75% B at a flow rate of 1.0 ml/min. The column temperature was maintained at 28°C and the injection volume was 10 μL (Lee et al., 2018 (link)). Chemicals used as a standard are Forsythoside A, phillyrin, and phillygenin. The constituents were identified by comparison of their retention times to those of standard compounds under identical analysis conditions and the UV spectra with our in-house DAD library.