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Pe or apc

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PE or APC are fluorescent labels used in flow cytometry applications. They are used to detect and quantify specific proteins or cell surface markers on cells. PE and APC provide bright fluorescence signals that can be detected by flow cytometers.

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Lab products found in correlation

Lsrfortessa x 20, by BD (2 mentions) Zombie nir fixable viability kit, by BioLegend (2 mentions)

Close spelling variants of this product

Anti-FOXP3-APC or PE (2 citations) Streptavidin conjugated PE or APC (2 citations)

2 protocols using pe or apc

1

Multiparameter Flow Cytometry for MR1 and HSV-1 Protein

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For flow cytometry staining of MR1 in HFFs the mAb 26.5 conjugated to Biotin in combination with Streptavidin conjugated to PE or APC (eBioscience) was utilized. In MR1 overexpressing cells 26.5 directly conjugated to PE (Biolegend) was used. Surface MHC I expression was detected by anti-HLA-A,B,C-PE (Miltenyi Biotec) or anti-HLA-A,B,C-APC (BD Biosciences). HSV-1 gD surface expression was detected using anti-gD-FITC (Virostat). Live cells were identified using Zombie NIR Fixable Viability Kit (Biolegend). Flow cytometry was performed using a LSR Fortessa X-20 (BD Biosciences), with data analyzed using FlowJo software (Treestar Inc.).
McSharry B.P., Samer C., McWilliam H.E., Ashley C.L., Yee M.B., Steain M., Liu L., Fairlie D.P., Kinchington P.R., McCluskey J., Abendroth A., Villadangos J.A., Rossjohn J, & Slobedman B. (2020). Virus-Mediated Suppression of the Antigen Presentation Molecule MR1. Cell reports, 30(9), 2948-2962.e4.
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2

Multiparameter Flow Cytometry for MR1 and HSV-1 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry staining of MR1 in HFFs the mAb 26.5 conjugated to Biotin in combination with Streptavidin conjugated to PE or APC (eBioscience) was utilized. In MR1 overexpressing cells 26.5 directly conjugated to PE (Biolegend) was used. Surface MHC I expression was detected by anti-HLA-A,B,C-PE (Miltenyi Biotec) or anti-HLA-A,B,C-APC (BD Biosciences). HSV-1 gD surface expression was detected using anti-gD-FITC (Virostat). Live cells were identified using Zombie NIR Fixable Viability Kit (Biolegend). Flow cytometry was performed using a LSR Fortessa X-20 (BD Biosciences), with data analyzed using FlowJo software (Treestar Inc.).
McSharry B.P., Samer C., McWilliam H.E., Ashley C.L., Yee M.B., Steain M., Liu L., Fairlie D.P., Kinchington P.R., McCluskey J., Abendroth A., Villadangos J.A., Rossjohn J, & Slobedman B. (2020). Virus-Mediated Suppression of the Antigen Presentation Molecule MR1. Cell reports, 30(9), 2948-2962.e4.
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