Mda kit

The concentration of Myeloperoxidase (MPO), Malondialdehyde (MDA), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GSH-Px) in mouse serum represents the level of oxidative stress in mice. After collecting the mouse serum, the manufacturer’s instructions given on the MPO kit (R&D Systems, Emeryville, CA, USA), MDA kit (R&D Systems, Emeryville, CA, USA), SOD kit (R&D Systems, Emeryville, CA, USA) and GSH-Px kit (R&D Systems, Emeryville, CA, USA) were followed to detect the oxidative stress level in the mouse.

MDA tests were conducted to measure the level of lipid peroxidation by an MDA kit R&D Systems, USA). Briefly, the fitted standard curve was established to determine the levels of MDA concentration. Then, the ipsilateral brain tissues around hematoma on day 7 were collected for lysis and centrifuge. Supernatants were firstly underwent acidification and then centrifuged for 3 times. Afterward, the samples were mixed with TBARS reagent for 2-3 h at 40-50 °C. The optic density (OD) value was determined at 532 nm using a spectrophotometer (Varioskan Flash, Thermo Scientific, Waltham, MA, USA); then, the MDA concentration was calculated by the fitted standard curve.