Hek 293t ace2h cells

HEK-293T cells, RAW264.7 cells, and the African green monkey epithelial (Vero E6) cells were supplied by American Type Culture Collection (ATCC, Rockville, MD, United States). HEK-293T-ACE2^h cells were constructed by Sino Biological (Beijing, China). All the cell lines were cultured in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% Gibco penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Waltham, United States) at 37°C in a humidified atmosphere of 5% CO₂. Besides, 100 μg/mL hygromycin was used for the culture of HEK-293T-ACE2^h cells. SARS-CoV-2 (Genebank accession no. MT123290.1) was clinical isolate from the First Affiliated Hospital of Guangzhou Medical University. The viral titer was determined by 50% tissue culture infective dose (TCID₅₀) according to the cytopathic effect by use of Reed-Muench method. All the infection experiments of SARS-CoV-2 strain were performed in a biosafety level-3 laboratory.

HEK-293T cells, LO2 cells, Beas-2B cells and HEK 293 cells were purchased from American Type Culture Collection (ATCC, Bethesda, MD, USA). HEK-293T cells highly expressing ACE2 (HEK-293T-ACE2^h cells) were constructed by Sino Biological (Beijing, China). Dulbecco modified eagle medium (DMEM), Opti-MEM, fetal bovine serum (FBS) and penicillin-streptomycin-glutamine mixture were obtained from Gibco (Grand Island, NY, USA). All the cell lines were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin-glutamine mixture at 37 °C in humidified air containing 5% CO₂. In addition, hygromycin B was used for the HEK-293T-ACE2^h cell culture media at a concentration of 100 µg/mL.

HEK-293T cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). HEK-293T-ACE2^h cells were constructed by Sino Biological (Beijing, China). All the cell lines were cultured in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, the antibiotics penicillin (50 U/mL) and streptomycin (50 μg/mL, Invitrogen, Paisley, Scotland, UK) at 37 °C in a humidified atmosphere of 5% CO₂. Besides, 100 μg/mL hygromycin was used for the culture of HEK-293T-ACE2^h cells.